3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09.07.2019 - 19.09.2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane

EC Number:

202-928-3

EC Name:

3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane

Cas Number:

101-25-7

Molecular formula:

C5H10N6O2

IUPAC Name:

3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the start of the exposure and at the renewal (0 and 24 hours), monitoring samples of the fresh media were taken after preparation of the limit concentration and analyzed.
At the renewal and at the end of the exposure (24 and 48 hours), samples of the 24-hours old media were taken directly from the test vessels and analyzed.

Test solutions

Vehicle:

no

Details on test solutions:

The study was conducted under semi-static conditions over a period of 48 hours with the limit concentration of 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane. The limit concentration was treated with ultrasound for 5 minutes at room temperature. The limit concentration was visually clear throughout the exposure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, obtained from continuous laboratory cultures.
Origin Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
Breeder Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany

Culture In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20  2 °C, in an incubator, 16 hours illumination; light
intensity of max. 1500 lx

Culture medium Elendt M4, according to OECD 202, Annex 3 (2004) is used.
Feeding of the culture stocks The daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella
subcapitata and Desmodesmus subspicatus. The algae were cultured at the test facility.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

224 - 267 mg CaCO3 /L

Test temperature:

18 - 22 °C, constant within ± 1 °C

pH:

7,5 - 8,86

Dissolved oxygen:

8.41 mg/L - 8,98 mg/L

Conductivity:

564 - 600 µS/cm

Nominal and measured concentrations:

Sampling date 0 hours 24 hours 24 hour 48 hours
Fresh medium old medium fresh medium old medium

Nominal
test item
concentration Meas. conc. Meas. conc. Meas. conc. Meas. conc.
[mg/L] [mg/L] % [mg/L] % [mg/L] % [mg/L] %
110 110 100 103 94 109 99 105 95
Control < LOQ < LOQ < LOQ < LOQ

Meas. conc. measured concentration of the test item, dilution and weighing factor of analytical stock solution taken into
account
% percent of the nominal concentration of the test item
LOQ limit of quantification (0.100 mg test item /L)

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 110 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Immobilization Rates after 24 and 48 hours of Exposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)

Test substance (110 mg/L) 0 of 20
Control 0 of 20

Results with reference substance (positive control):

The percentage of immobility for the reference item potassium dichromate (SIGMA-ALDRICH, batch number MKBV0900V, purity 99.0%, CAS RN 7778-50-9) was determined after 24 hours from 2019-07-17 to 2019-07-18.

EC50 1,95 mg/L
95% confidence limits 1,00 - 4,00 mg/L
Valid range 0.6 - 2.4 mg/L, acc. to AQS P 9/2 (02/2000)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In the limit concentration 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane, no effects on Daphnia magna were observed.

Executive summary:

In the limit concentration 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane, no effects on Daphnia magna were observed.