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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
other: read across from similar substance
Adequacy of study:
key study
Study period:
2001
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to
Guideline:
other: Test No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters."
Deviations:
no
Principles of method if other than guideline:
- Principle of test:
A sample of the tested substance was dissolved in the dilution water at a concentration of 0.1 g/L. The total volume for one dilution is 1000 ml for preliminary as well as for full test. There were used 7 dilution of the test substance.

- Short description of test conditions:
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.
Test duration 96 hours, the temperature of the solutions 22.0 ± 1.0 °C.

- Parameters analysed / observed:
mortality at 48 and 96 h

GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Guppy
- Strain: Poecilia reticulata
- Source: AKVA-SALON, Žižkova 977, 535 01 Přelouč
- Age at study initiation (mean and range, SD): 3 - 4 monts
- Length at study initiation (length definition, mean, range and SD): 2 - 3 cm

ACCLIMATION
- Acclimation period: yes
- Acclimation conditions (same as test or not): same as test

FEEDING DURING TEST
no feeding during test
fasted 72 hours before test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
22.0 ± 1.0 °C
pH:
7.8 (for measured values see attached document with tables)
Dissolved oxygen:
5.40 - 8.6 mg/L O2 (see attached document with tables)
Nominal and measured concentrations:
100.04 mg.L-1
Details on test conditions:
TEST SYSTEM
- Material, size, headspace, fill volume: open 10 L glass aquarium
- Aeration: without
- No. of organisms per vessel:
Preliminary test: 3 animals
Full test: 10 animals
- No. of vessels per concentration/control (replicates): no replicates

TEST MEDIUM / WATER PARAMETERS
Dilution water: standard dilution water was prepared by mixing 2.5 ml of stock solutions (A,B,C,D) with 1000 ml distilled water
A) 117.6 g CaCl3.2H2O in 1L of distilled water
B) 49.3 g MgSO4.7H2O in 1L of distilled water
C) 25.9 g NaHCO3 in 1L of distilled water
D) 2.3 g KCl in 1L of distilled water
The dilution water should have a pH of 7.8 and is saturated with oxygen at a temperature 20 – 24°C.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality at 48, 96 h

TEST CONCENTRATIONS
- Range finding study: 100, 75, 50, 25 mg.L-1
- Test concentrations: 100, 90, 80, 70, 60, 50, 40 mg.L-1
- Results used to determine the conditions for the definitive study: see attached document with tables
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
77.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
yes
Remarks:
The measured values of pH solution not change more than 1 pH unit.
Executive summary:

The test substance was tested for acute toxicity on fish Poecilia reticulata. The test was performed according to method No. L 47 "Fish, Acute Toxicity test. Chemical formulations and waste waters".

Results:

48 h - LC50 = 80.0 mg.L-1

96 h - LC50 = 77.3 mg.L-1

Description of key information

LC 50 96h = 77,3 mg/l

Key value for chemical safety assessment

LC50 for freshwater fish:
77.3 mg/L

Additional information

The result of the acute toxicity tests showed some effects to fish.

The species used in the key test was Poecilia Reticulata and the concentration were: 100, 75, 50 and 25 mg/l.

The results of the test were:

- 48h LC50 = 80 mg/l
- 96h LC50 = 77,3 mg/l
The presence of toxic effects for fish is confirmed by the results of the supporting study.

Based on the criteria of the Regulation 1272/2008, the substance needs to be classifies as Aquatic Chronic 3, H412.