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EC number: 811-285-3 | CAS number: 1637294-12-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- NYMPHEAL (GR-88-0778) :
Lot No. : Batch 6
Purity : 99.1% (sum of the isomers)
Date of Expiry : 29 March, 2017 - Analytical monitoring:
- no
- Remarks:
- A dissolved oxygen measuring device composed of a flat bottom flask (BOD type, Karlsruher Flasche) in which an O2-electrode (StirrOx G, WTW, Weilheim, Germany) fits tightly and an oxygen-meter (inoLab Oxi 730, WTW, Weilheim, Germany).
- Vehicle:
- no
- Details on test solutions:
- Test Procedure :
Control C1: at time "0 minute", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Control C2: at time "15 minutes", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Control C3: at time "30 minutes", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Test S1: at time "45 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 50 mg of test substance were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final test substance concentration: 100 mg/l.
Test S2: at time "60 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 50 mg of test substance were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final test substance concentration: 100 mg/l.
Test S3: at time "75 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 50 mg of test substance were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final test substance concentration: 100 mg/l.
Test R1: at time "90 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 1 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration:
2.00 mg/l
Test R2: at time "105 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 1 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration: 2.00 mg/L.
Test R3: at time "120 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 1 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration: 2.00 mg/l
Test R4: at time "135 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 12.5 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration: 25.00 mg/l
Test R5: at time "150 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 12.5 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration: 25.00 mg/l
Test R6: at time "165 minutes", in another 500-ml cylinder, 16 ml of the synthetic sewage feed + 12.5 mg of 3,5-dichlorophenol were diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated. Final reference substance concentration: 25.00 mg/l
Control C4: at time "180 minutes", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Control C5: at time "195 minutes", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Control C6: at time "210 minutes", in a 500-ml scaled cylinder, 16 ml of the synthetic sewage feed was diluted to 250 ml with water. 250 ml of sludge were added and the mixture was aerated continuously for 3 hours by bubbling air into it.
Measurements of the oxygen consumption: at time "3 hours", the pH of the first cylinder C1 was measured. The content was used to fill up the BOD flask which was closed with the O2-electrode and the measurement of the oxygen consumption was started for about 10 minutes. The same procedure was repeated every 15 minutes for each cylinder whit bubbling air in the order they had been prepared, so that the contact time is three hours for each of them.
The whole experiment was carried out at 20 ± 2°C (temperature controlled water bath). - Test organisms (species):
- other: synthetic sewage feed
- Details on inoculum:
- Synthetic sewage feed :
A synthetic sewage feed was made by dissolving the following amounts of substances in 1 litre of tap water:
- 16.0 g peptone (from casein trypsin-digested)
- 11.0 g yeast extract powder
- 3.0 g urea
- 0.7 g NaCl
- 0.4 g CaCl2 · 2 H2O
- 0.3 g MgSO4 · 7 H2O
- 2.8 g K2HPO4
Activated sludge :
Activated sludge was obtained from a water treatment plant treating predominantly domestic sewage (Bois-de Bay, 1242 Satigny, Switzerland). The sludge was kept aerobic until being used on the next day. The pH was 7.5.
The suspended solids content was determined by drying 50 ml of the sludge and weighing: dry weight of suspended solids: 5.00 g/l.
By adding 1670 ml of water to 2500 ml sludge, a concentration of suspended solids of 3.0 g/l was obtained. With the final dilution during the test, a concentration (i.e. in the range 0.8 to 1.6 g/l as recommended in ISO 8192-1986) was obtained. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- 3,5-dichlorophenol was used as a reference substance to check the activity of the sludge: its EC50 ( 3 hours) should be withi the range of 2 to 25 mg/L.
Observed :
2.0 mg/L 0.9 to 8.7 % inhibition of respiration
25.0 mg/L 72.6 to 79.1% inhibition of respiration - Validity criteria fulfilled:
- yes
- Conclusions:
- An activated sludge respiration inhibition test was performed with GR-88-0778. In a limit test a test concentration of 100 mg/l was tested.
The result can be expressed as follows: The EC50 (3 hours) of GR-88-0778 is > 100 mg/L.
Reference
Description of key information
An activated sludge respiration inhibition test was performed with GR-88-0778. In a limit test a test concentration of 100 mg/l was tested. The result can be expressed as follows:
The EC50 (3 hours) of GR-88-0778 is > 100 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 mg/L
Additional information
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