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EC number: 701-347-7 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErC50 (72h) > water solubility limit
NOEC (72h) > water solubility limit
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
The toxicity of the substance to aquatic algae is assessed considering data on similar substances in a Weight-of-Evidence approach. Justification for Read Across is given in Section 13 of IUCLID. Two studies are available conducted according to the OECD Guideline 201.
In the first study performed also under GLP conditions, static conditions were used and the algae were exposed to 5 mg/L of the substance (limit test) in triplicate. A solvent was used to prepare the test solution due to low water solubility and the limit test at 5.00 mg/L was performed in order to keep the recommended concentration of vehicle (i.e. 100 mg/L in the final test solution). Solvent and vehicle controls run in parallel in triplicate. Analytical monitoring was done at the start and at the end of the test. Since the measured concentration was between ± 20 % of the nominal concentration, the results are based on nominal concentrations. The control response was satisfactory.
There was no statistical significant difference in inhibition rate compared with the vehicle control and both EC50 (72h) and NOEC (72h) were greater than the concentration used in the limit test.
ErC50 > 5.00 mg/L (24-72 h);
NOEC(r) > 5.00 mg/L
EbC50 > 5.00 mg/L
(0-72 h); NOEC(b) > 5.00 mg/L
The second study has a lower reliability as not a lot of details are available. Desmodesmus subspicatus were exposed to the test substance for 74 hours at a single nominal concentration of 10 mg/L (highest concentration tested based on limited water solubility; Union Camp, 1993c). The NOEC was >=10 mg/L and the 72 hour EbC50 and 24 hour ErC50 were >10 mg/L.
In both studies it seems that no growth inhibition of algae was observed up to the highest tested concentration which would be the water solubility limit of each substance. For this reason, the Key Information is provided as > 100 mg/l which is the upper limit of classification for aquatic toxicity as per the CLP Regulation EC No. 1272/2008.
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