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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study and GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Principles of method if other than guideline:
Guidelines:

- Commission Regulation (EC) No 440/2008, Annex, Part C, C.2.: "Daphnia sp. Acute Immobilisation Test", Official Journal of the European Union (EN), dated May 30, 2008

- OECD Guideline for Testing of Chemicals 202: "Daphnia sp., Acute Immobilisation Test" adopted April 13, 2004

- OECD Series on Testing and Assessment, No. 23, "Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures", December 15, 2000
GLP compliance:
yes (incl. QA statement)

Test material

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.578 mg/L
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
LOEC
Effect conc.:
0.612 mg/L
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.11 mg/L
Nominal / measured:
meas. (geom. mean)
Basis for effect:
mobility
Remarks on result:
other: 95 % confidence values = 0.973 - 1.29 mg/l

Any other information on results incl. tables

The quantification of the test item Sa 57 (SAT No. 090073) was performed using gas chromatography (GC-MS-method).

At the start of the test 108 % of the nominal test concentration was found (geometric mean value of influent and effluent recoveries). After 24 hours test duration, 64 % of the nominal value was determined (geometric mean value of influent and effluent recoveries). At the end of the test at 48 h 76 % of the nominal test concentration was found (geometric mean value of influent and effluent recoveries).

A significant decrease of the test item occurred between the in- and efflux of the flow-through test assembly. Therefore, the effect assessment is based on the geometric mean concentrations of influx and efflux at the respective time.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The toxic effect of the test item Sa 57 (SAT No. 090073) to Daphnia magna was assessed in a dose-response-test. Due to the low water solubility and the degradation properties of the test item in water, the test item was diluted in ethanol and a flow-through test was performed. Since the test item formed undissolved droplets on the water surface of the aquaria, cages were introduced in the aquaria to ensure that the test item has no physical effect on the daphnids. The 48-hour NOEC was determined to be 0.578 mg test item/L. The 48-hour LOEC was determined to be 0.612 mg test item/L The 48-hour EC50 value was calculated to be 1.11 mg test item/L.
Executive summary:

After 48 hours of exposure no immobilisation of the test animals was observed in the blank control, the solvent control and the test item concentration of 0.578 mg/L. At the concentration of 0.612 mg/L, 2/20 animals were immobile and 9/20 animals were immobile at the concentration of 1.08 mg/L. At the highest test item concentrations of 1.63 and 3.01 mg/L, 17/20 and 20/20 daphnids were immobile, respectively.