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EC number: 479-660-0 | CAS number: -
A respiration inhibition test with activated sludge according to OECD guideline 209 was performed for 3 with the test substance. Test system was activated sludge of the municipal treatment plant of Sarstedt, Germany. The test was performed under static conditions as limit test with the concentration: 1000 mg/L. The respiration rates of control, reference and test item concentrations were measured after a contact time of 3 h, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. An inhibition of 10% was observed at the tested limit concentration. In order to check the activity of the test system and the test conditions a reference test is carried out with copper(II) sulphate pentahydrate as reference item and the reference toxicity is detewrmined. The EC50 -value for the reference item was 124 mg/L. Validity criteria of the test guideline were fulfilled.
EC50 (test item): > 1000 mg/L
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