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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1999
Deviations:
yes
Remarks:
no test item analysis possible
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Dec. 1992
Deviations:
yes
Remarks:
no test item analysis possible
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Due to the insolubility of the test item no concentration-control analysis could have been carried out. Therefore, no information on stability and recovery of the test itemunder test conditions were given.

Test solutions

Vehicle:
yes
Remarks:
dilution water
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
An appropriate amount of test item was crushed with a mortar. The solution (100 mg/L test item was weighed out) was prepared with dilution water one day prior to application. the stock solution was shaken with 20 rpm for 24 h and membrane filtered with 0.45 µm.

Dilution water: according to EC 92/69 L383A C.2. Annex

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Justification for species other than prescribed by test guideline: n.a.
- Source:
a. Origin: Institut für Wasser-, Boden- und Lufthygiene
b. Breeder: Dr. Noack-Laboratorium, Sarstedt, Germany
- Age of test organisms: 2-24 h
- Feeding during test: none
during culture:
- Food type: mix of Desmodesmus subspicatus and Chlorella vulgaris
- Amount: lgae densitiy of > 10(exp6) cells/mL
- Frequency: at least 5x weekly, ad libitum

ACCLIMATION
- Acclimation period: ca. 2 h in dilution water
- Type and amount of food: none
- Feeding frequency: n.a.
- Health during acclimation (any mortality observed): no


Study design

Test type:
static
Water media type:
other: dilution water according to EC 92/69 L383A C.2. Annex
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
CaCO3: 268 mg/mL
Test temperature:
19 +/- 1°C
pH:
Test item:
- study beginning: 7.99
- study end: 7.6

Control:
- study beginning: 7.89
- study end: 7.72
Dissolved oxygen:
Test item:
- study beginning: 9.14 mg/L
- study end: 8.73 mg/L

Control:
- study beginning: 8.84 mg/mL
- study end: 8.2 mg/mL
Salinity:
according to EC 92/69 L383A C.2. Annex
Conductivity:
676 µS/cm
Nominal and measured concentrations:
saturated solution as limit concentration
Saturation concentration: The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium acc. to OECD
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beakers (5 cm diameter x 8 cm height), 50 mL volume
- Fill volume: 20 mL
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): n.a.
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to EC 92/69 L383A C.2. Annex
- Total organic carbon: 268 mg CaCO3/mL
- Conductivity: 676 µS/cm
- Culture medium different from test medium: Elendt M4 modified to a total hardness of 160 to 180 mg/ CaCO3/L
- Intervals of water quality measurement: at the study start and after 48 h

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 15/8 h light/dark cycle
- Light intensity: diffuse light, illumination range max. 20 µmol x m(-2) x s(-1)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 1:1, 1:10 and 1:100 dilution of the saturated solution
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: saturated solution/saturation concentration; the maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium according to OECD. 100 mg/L weighed out for preapration of the saturation concentration
Key result
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: saturated solution/saturation concentration; the maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium according to OECD. 100 mg/L weighed out for preapration of the saturation concentration
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: saturated solution/saturation concentration; the maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium according to OECD. 100 mg/L weighed out for preapration of the saturation concentration
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: saturated solution/saturation concentration; the maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium according to OECD. 100 mg/L weighed out for preapration of the saturation concentration
Key result
Duration:
24 h
Dose descriptor:
EC10
Effect conc.:
0.58 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
1.49 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
5.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

There is no biologically significant effect neither in the tested saturation concentration nor in the control group.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
At the saturation concentration of the test substance (i.e. the maximum dissolved concentration of the test item that can be achieved under the test consitions in the test medium according to OECD guideline) no biologically significant effect was determined.
Executive summary:

In the acute immobilisation test with Daphnia magna (STRAUS) the effcet of the saturated solution (i.e. the maximum dissolved concentration of the test item that can be achieved under the test consitions in the test medium according to OECD guideline) as limit concentration of the test item was determined accodring to OECD guideline 202. The test item was clearly dissolved in the saturation concentration. 100 mg/L were weighed out for the preaparation of the saturation concentration. The study was conducted under static conditions over a duration of 48 h. 20 test organisms were exposed to the saturation concentration and control. The limit concentration was not analytically verified, due to the insolubility of the test item. Effect values are given based on the nominal concentration. A reference test is carried out once per month with potassium dichromate to determine the reference toxicity. The EC50 -value of the reference item at 1.49 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within acceptable limits.

The validity criteria of the test guideline were fulfilled.