Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From January 12, 2018 to March 01, 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: Daphnia magna was obtained from the CAROLINA BIOLOGICAL SUPPLY COMPANY - Burlington, North Carolina (USA) and maintained as a stock culture at BIOAGRI.
Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h
Test temperature:
20.1 to 20.3°C
pH:
7.7 to 8.3
Dissolved oxygen:
7.8 to 8.1 mg/L
Nominal and measured concentrations:
Nominal concentrations for definitive test: 0.65; 1.3; 2.5; 5 and 10 mg/L.
Details on test conditions:
- Test volume: 15 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 replicates for per test concentration a
- No. of vessels per control (replicates): 4
- Photoperiod: 16 hours of light and 8 hours of dark
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
The 24h-EC50 of reference substance (potassium dichromate) was 1.05 mg/l.

Table 1. Number of Immobile Organisms at 24 and 48 Hours of Exposure, During the Definitive Test.

Test concentration (nominal)

(mg/L)

Number of immobile organisms

24 hours

48 hours

a

b

c

d

% of total immobility

a

b

c

d

% of total immobility

10

5

5

5

5

100

5

5

5

5

100

5

3

2

2

2

45

4

2

3

2

55

2.5

1

1

0

1

15

1

2

2

1

30

1.3

0

0

0

0

0

0

0

0

0

0

0.65

0

0

0

0

0

0

0

0

0

0

Control

0

0

0

0

0

0

0

0

0

0

a, b, c and d = replicates with 5 organisms each.

 

Table 2. Number of Immobile Organisms at 24 Hours of Exposure, During the Test with the Reference Substance.

Concentration

(mg/L)

Number of immobile organisms

24 hours

a

b

c

d

% of total immobility

2.4

5

5

5

5

100

1.8

5

4

5

4

90

1.0

3

3

2

2

50

0.56

0

0

0

0

0

0.32

0

0

0

0

0

Control

0

0

0

0

0

a, b, c and d = replicates with 5 organisms each.

 

Table 3. Dissolved Oxygen and pH of the Tested Concentrations and Control, at the Beginning and at the End of the Definitive Test.

Nominal concentrations

(mg/L)

pH

Dissolved oxygen (mg/L)

0 hour

48 hours

0 hour

48 hours

10

8.2

8.3

8.1

7.9

5

8.1

8.2

8.0

7.9

2.5

7.9

8.0

7.9

7.7

1.3

7.9

8.0

8.0

7.8

0.65

7.8

8.0

8.0

7.8

Control

7.7

7.9

8.1

7.9

Validity criteria fulfilled:
yes
Conclusions:
According to OECD 202 test method, the 48-hr EC50 of Everzol SR98 was 3.95 mg/L.
Executive summary:

This test using the procedures outlined in the BIOAGRI Study Plan for 16264.206.071.17 and OECD 202 (OECD, 2004). The results of this OECD 202 test for Everzol SR98 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of Everzol SR98 was 3.95 mg/L.

Description of key information

The 48-hr EC50 of Everzol SR98 was 3.95 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
3.95 mg/L

Additional information

Not more than 10 % of the Daphnia were immobilised in the control and the dissolved oxygen concentration at the end of the test was ≥3 mg O2/L. All test validity criteria were met. The values of pH of the control and test solutions measured at 0 and 48 hours not deviate more than 1.5 units during the test. The 24-hours EC50 value of the reference substance (potassium dichromate) was 1.05 mg/L, showing the sensitivity of the test system. Under the conditions of this test, the 48h-EC50 of Everzol SR98 was 3.95 mg/L.