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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test item attained 37 % biodegradation after 28 days and 42 % biodegradation after 36 days and cannot be considered readily biodegradable (OECD 301B, EU Method C4 -C and US EPA OCSPP 835.3110).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

GUIDELINE

A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 301B "Ready Biodegradability; CO2 Evolution Test referenced as Method C4 -C of Commission Regulation (EC) No 440/2008 and US EPA Fate, Transport and Transformation Test Guidelines OCSPP 835.3110 (Paragraph (m)).

METHODS

The test item, at a concentration of 10 mg carbon/L, was exposed to activated sewage sludge microorganisms with mineral medium in sealed culture vessels in the dark at temperatures between 20 and 25 °C for 35 days. The study was extended from 28 days to 35 days at the request of the sponsor. Biodegradation of the test item was assessed by determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, were used for validation purposes together with a toxicity control.

RESULTS

The test item attained 37 % biodegradation after 28 days and 42 % biodegradation after 36 days. The test material cannot therefore be considered readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.