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EC number: 291-909-3 | CAS number: 90506-47-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- The temperature in the experiment was in the range of 21 .8 - 25.5 °C and therefore higher than stated in the study plan. Because normal growth was observed, this can be stated as uncritical.
Due to a technical accident only 182 mL instead of 200 mL test solution was used for preparation of treatment 32 mg/L. As the amount of algae added to the solution was adjusted to the amount of test solution and the extinction was in the expected range at the start of the test, this can be stated as uncritical.
Considering that these deviation are uncritical, the study is considered without significant deviation. - Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine
- EC Number:
- 291-909-3
- EC Name:
- Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine
- Cas Number:
- 90506-47-1
- Molecular formula:
- complex substance
- IUPAC Name:
- Phosphoric acid, C8-16-alkyl esters, reaction products with (Z)-N-9-octadecenyl-1,3-propanediamine
- Test material form:
- liquid: viscous
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and batch No.of test material: ARCOT 3135 from PCAS, Batch N° 80555C079
- production date: May 2015
- date of receipt: 08 June 2015
- Expiration date of the batch: May 2016
- Purity test date: 28 May 2015
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: room temperature at room temperature (19.2 - 24.0 °C), in proper conditions in a closed vessel.
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The water-accommodated fractions (WAFs) were prepared for the test. This was done mixing the nominal loads with the corresponding amount of nutrient medium (demineralised water enriched with minerals but without algae) and shaking vigorously for 24 hours. The resulting solutions were filtrated through 0.45 µm nylon filters.
- Controls: Positive control used: potassium dichromate K2Cr207 (CAS No. 7778-50-9) was used as positive control in a separate reference test. Blank control: deionised water with nutrient medium and algae
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: freshwater green alga
- Genus: Desmodesmus
- Species: subspicatus
- SAG Strain Number: 86.81
- Source: The culture of Desmodesmus subspicatus was obtained in February 2014 by MBM Sciencebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen).
- Method of cultivation: kept as stock culture on solid agar at 2-8 oc. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared.
ACCLIMATION
- Acclimation period: 96 hours
- Culturing media and conditions: Four days before the start of each test, an aliquot of the permanent culture was brought into pre-culture medium and incubated under continuous lighting for 96 hours. The resulting culture grew exponentially. Before usage, the pre-culture was checked for the absence of cell aggregates and the cell number of culture was determined via photometric measurement.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- As recommended in the OECD Guideline.
Test conditions
- Test temperature:
- 21.8 - 25.5 °C
- pH:
- 7.7 - 7.8
- Nominal and measured concentrations:
- 1.0 / 3.2 / 10 / 32 / 100 mg/L nominal concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass flasks total volume 65 mL
- Type: open
- Material, size, headspace, fill volume:
- Initial cells density: cell concentration of approximately 5*10^3 cells/mL
- No. of vessels per concentration (replicates): three replicates for each treatment
- No. of vessels per control (replicates): six replicates for the control
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: yes, varying between 7.7 - 7.8
- Light intensity and quality: 5300 lux during all the study.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: The cell numbers were determined by photometric measurement of optical density.
TEST CONCENTRATIONS
- Test concentrations: 1.0 / 3.2 / 10 / 32 / 100 mg/L nominal concentration - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- Yield
- Reported statistics and error estimates:
- The estimation of the biological data was accomplished using the software ToxRat@ Professional, version 3.1 The details of calculation are stated in the annex, page 29 of the complete study report attached to the Iuclid file. Biological results were stated using two significant digits.
Any other information on results incl. tables
From the cell numbers, the Growth Rate and the Yield were calculated. The means and standard deviations at the end of the test are given in the following table:
Nominal Concentration in mg/L |
|
Growth Rate (0-72h) [day-1] |
Yield (0-72h) [Cell Concentration/mL] |
Blank control |
Mean |
1.38 |
332743 |
SD |
0.04 |
37397 |
|
1.0 mg/L |
Mean |
1.39 |
351428 |
SD |
0.06 |
57882 |
|
3.2 mg/L |
Mean |
1.26 |
231051 |
SD |
0.03 |
18337 |
|
10 mg/L |
Mean |
0.89 |
74382 |
SD |
0.08 |
18389 |
|
32 mg/L |
Mean |
0.37 |
11499 |
SD |
0.10 |
4861 |
|
100 mg/L |
Mean |
0.25 |
7187 |
SD |
0.19 |
7178 |
SD = Standard Deviation
Applicant's summary and conclusion
- Conclusions:
- The 72h ErC50 is 17 mg/L (95% confidence interval 14 - 20 mg/L)
The 72 EyC50 is 5.1 mg/L (95% confidence interval 4.4 - 5.8 mg/L) - Executive summary:
A study was conduct for the Determination of the toxicity of ARCOT 3135 in Desmodesmus subspicatus according to OECD 201 resp.EU C.3. The study was performed using 5 concentrations ranging from 1 .0 to 100 mg/L nominal concentration. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the absorption of the solutions at 440 nm every 24 hours with a spectral photometer. The cell density of the cultures was calculated based on the correlation curve between the absorption and the cell density of the cultures determined by with a cell counter. Growth rate and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations: 100 / 32/ 10/3.2 mg/L. The following results for the test item ARCOT 31 35 were determined:
The 72h ErC50 is 17 mg/L (95% confidence interval 14 - 20 mg/L)
The 72 EyC50 is 5.1 mg/L (95% confidence interval 4.4 - 5.8 mg/L)
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