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EC number: 840-567-9 | CAS number: 2247629-85-0
1. The protocol states that the total dissolved oxygen will not be allowed to drop below 60% or exceed 105% of saturation for the duration of the test. At the 0-hour interval, the total dissolved oxygen was approximately 110% of saturation in all treatment levels tested. Because control survival exceeded acceptable criteria and no adverse effects were observed in the controls, this deviation did not have a negative effect on the results or the interpretation of this study.
2. The protocol states that QC samples will be prepared at each sampling interval and stored and analyzed with the set of study samples. Because iron is stable in aqueous solutions, the QC samples were prepared at test termination in order to assess the effects of shipping on stability. The test solution analyses and the QC sample results were within acceptable recovery range (80 to 120% of nominal), therefore this deviation did not have a negative impact on the results or interpretation of the study.
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