Absolute of Nicotiana tabacum (Solanaceae) obtained from leaves by organic solvent treatment and subsequent ethanol extraction

Endpoint summary

Description of key information

The substance belongs to a group of Natural Complex Substances (NCS): UVCB sub-type 3, where the source is biological and the process is refinement (See ECHA Guidance on Identification and naming of substances under REACH); such as: exudates, resinoids and concretes that are well-known to be very difficult to analytically assess. These complex UVCB substances contain a large number of constituents with different volatility and solubility (mostly low solubility); the composition can be widely unknown and being (mostly) solid(ish) and/or extremely viscous resins their physical state leads to further difficulties for testing.

 

Considering the very complex nature of the test item (UVCB, Viscous liquid, mainly composed of unknown constituents), physico-chemical properties of the registered substance were determined from experimental testing when possible. Some endpoints were waived, based on the experience in handling, testing and use of the substance and on the chemical structure of the known constituents. We also considered waiving based on the technical issues due to the complex nature of such UVCB substance especially when the information obtained from testing was not relevant or of practical use and irrelevant for subsequent assessments.

 

Based on available data, and according to both GHS and CLP criteria, the substance is NOT classified for physical hazards (but the dataset is incomplete). The substance is described as a viscous liquid, so Aspiration Hazard will Not apply.

Additional information

Summary of relevant physico-chemical properties:

 

Endpoint	Data
Appearance	The appearance of the test substance was stated as dark brown-yellow viscous liquid.
Melting point	The freezing/melting point is stated as: < - 20 °C (< 253.15 K).
Boiling point	The initial boiling point can be supposed to be between150 and 160 °C (dynamic method)
Relative density	Using the pycnometer method, a density of 0.9921 ± 0.0001 g/cm3 at 20.0 ± 0.4 °C was determined.
Vapour pressure	The vapour pressure of the test item was calculated as 516 Pa at 20.0 °C and 670 Pa at 25.0 °C.
Partition coefficient	Waived based on technical issue and poor knownledge of the composition of the substance + Estimation on known constituents of the registered substance.
Water solubility	Waived considering the very complex nature of the test item, the information obtained from testing is considered as not relevant or of practical use for subsequent assessments.
Surface tension	No structural alert, not a desired property.
Flash Point	The flash point is given as 98.3 ± 0.8 °C (corresponding to 371.4 ± 0.8 K).
Auto-Ignition Temperature	Auto-ignition temperature is stated as 270°C.
Flammability if contact with air or water	Waived based on experience in handling and use, and chemical structure of known constituents of the substance. NOT flammable if contact with air or water.
Self-heating	Waived based on physical state (self-heating unlikely for liquids, and method not applicable).
Explosive properties	Waiving based on experience on this type of substance and chemical structure of known constituents. Low potential for explosive properties.
Oxidising properties	Waiving based on chemical structure of known constituents of the substance. Registered substance is considered to have low potential for oxidising properties.

Slight variations are possible with composition (UVCB substance).