1,3,5-triazine-2,4,6(1H,3H,5H)-trithione

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-06-14 to 2006-06-16

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: "Method concerning tests relating to new chemical substances"

Version / remarks:

No.1121002 by the Pharmaceutical and Food Safety Bureau, 2003; No.2 by the Manufacturing Industries Bureau, 2003; No. 031121002 Environmental Health Department, Policy Division

Principles of method if other than guideline:

method similar to OECD202 performed under GLP; with analytical verification of test substance

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione

EC Number:

211-322-8

EC Name:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione

Cas Number:

638-16-4

Molecular formula:

C3H3N3S3

IUPAC Name:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All concentrations were sampled at test start and test end.
- Sample storage conditions before analysis: Instant analysis

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was prepared and diluted with dilution water for each concentration level. For the highest test concentration, the stock solution was used without dilution.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Ministry of the Environment, Institute for Environmental Studies, Japan; date of receipt: 04 August 1997
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Subculture breeding, maximum breeding period: 4 weeks
- Feeding during test: None

CULTURE
- Type and amount of food: P. subcapitata, 0.1 - 0.2 mg C per daphnia
- Feeding frequency: 3 - 5 days per week

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

200-250 mg/L (as CaCO3 equivalent)

Test temperature:

19.8° C - 20.4° C

pH:

6.5 - 7.8

Dissolved oxygen:

8.6 - 9.0 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0 mg/L, 6.3 mg/L, 12.5 mg/L, 25.0 mg/L, 50.0 mg/L, 100.0 mg/L
Measured concentrations (geometric mean after 48h exposure): < 0.15 mg/L, 6.0 mg/L, 12.4 mg/L, 25.0 mg/L, 50.3 mg/L, 102.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers
- Type (delete if not applicable): covered
- Material, size, fill volume: Glass, 100 mL, 80 mL
- Aeration: None, dilution water was aerated before test start (dissolved oxygen is more than 3 mg/L during exposure)
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M7 medium was used.
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, oxygen, pH and hardness were measured at test start and after 48 h.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: 800 - 1200 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Immobilisation was observed every 24 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 25, 50 and 100 mg/L (nominal), 25.6, 52.0 and 105.1 mg/L (measured)
- Results used to determine the conditions for the definitive study: EC50 (48h) 42.3 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

38 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL 31.6 to 45.7 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

52.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

1,3,5-triazine-2,4,6(1H,3H,5H)-trithione, trisodium salt (TMT)

Remarks on result:

other: recalculated value

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

50.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: No mortalities
- Immobilisation of daphnia after 48 h of exposure: 10% at 25 mg/L, 80% at 50.3 mg/L, 100% at 102.5 mg/L
- 0% immobilisation maximum concentration: 12.4 mg/L after 48 h; 100% immobilisation minimum concentration: 102.5 mg/L.
- Analytics: Measured concentrations ranged from 96.8 to 99.1% of nominal values at test start and from 95.2 to 106.1% of nominal values at test end. The concentration of the test material at the time of initial exposure was within ± 20% of the established concentration range. The test material concentration in the test liquid wa within ± 20% of the initial concentration during the exposure period (48 h).

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50 (48): 0.9 mg/L (95% CL: 0.74 - 1.10 mg/L)
- Date of experiment: 12 Mar - 14 Mar 2006

Reported statistics and error estimates:

EC50 values were calculated using the Probit method.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 after 48 hours incubation based on mobility on Daphnia magna was 38 mg/L (95% confidence interval: 31.6 to 45.7 mg/L).