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EC number: - | CAS number: -
Table 1: Cell counts
Nominal Test Concentration
(mg/L as WAF)
Mean Cell Counts 10⁴ cells/mL
* statistically significant inhibition (p≤0.05)
Table 2: Nominal and measured concentrations of the test solutions
Mean Measured Concentration
Results for 96 hours are from rederivatised samples, which confirmed the results from the original derivatised samples.
*0 hour nominal - 96 hour nominal. Variability in loss of measured concentration between hour 0 and hour 96 is believed to be related to sampling a water-accommodated fraction for a nonwater-soluble test material.# A gain was observed which may be attributed to the viscosity of the test substance and the method for sampling a water accommodated fraction for a nonwater-soluble test substance.
Table 3: Fortifications and recoveries of the test substance in quality control samples
Low Spike A*
Low Spike B*
Mid Spike A
Mid Spike B
High Spike A
High Spike B
The number of significant figures in the nominal concentrations of the spike samples varied due to the precision of the balance used to weigh the test material to prepare the spike samples.* Results were unacceptable high because the precision of the balance was to four decimal places and 0.0001 g of test material was weighed to prepare the low spike samples.
The toxicity of the test material to algae was evaluated using a test conducted in accordance with EPA OTS 797.1050 and OECD 201.
Cell growth was demonstrated in each test level at each time point. The test substance was considered to have an algistatic effect on cell growth. The multiple means test (Dunnett’s test) indicated a significant inhibition effect on growth at 0.80, 1.6, 3.2 and 6.4 mg/L WAF (nominal concentration) when compared to the controls at 96 hours.
The ErC50 values could not be determined because the maximum percent inhibition was not greater than 45 % for any time point.
The EbC50 (96 hours) for the test material based on nominal test concentrations was calculated to be 1.9 mg/L as WAF and based on mean measured concentrations, 1.3 mg/L. After 96 hours, the NOEC was 0.80 mg/L as WAF (nominal test concentration) and 0.89 mg/L (mean measured concentration) based on the absence of a growth inhibition effect.
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