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EC number: 613-895-3 | CAS number: 6609-57-0
A study was performed to assess the adverse effects of the test substance on the growth rate of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006).
A range finding test preceded the main test and provided information about the range of concentrations which were used in the main test. The following nominal concentrations of the test item were tested in the range finding test: 0.1, 1.0, 10 and 100 mg/L.
Exponentially growing algal cells were exposed for a period of 72 hours to a range of concentrations, 0.032, 0.10, 0.32, 1.0, 3.2, 10, 32 and 100 mg/L of test substance dissolved in dilution water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer.
During the test a temperature range of 21 - 24 °C was maintained in the test vessels. The pH was measured at the beginning of the test and after 72 hours of exposure.
In order to check whether or not significant amounts of the test item were incorporated into the algal biomass during the test period, a test flask at the highest test concentration without algae was run in parallel to the geometric series of test concentrations.
The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. Growth rates were also used to calculate a No Observed Effect Concentration (NOEC) and a Lowest Observed Effect Concentration (LOEC) according to Williams Multiple Sequential t-test Procedure. The following values were determined after 72 h: EC10 = 9.2 mg/L, EC50 = 26 mg/L.
The results are expressed in terms of nominal concentrations. Effective concentrations ranged from 98.9 % to 122 % of nominal values at 0 hours, and from 98.0 % to 107 % of nominal values at 72 hours.
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