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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug 30, 2018 - Jan 15, 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
Principles of method if other than guideline:
none
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
at 0 and 96 h
Vehicle:
no
Details on test solutions:
Dechlorinated drinking water sterilized by UV lamp is used in the test.
Test organisms (species):
other: Gobiocypris rarus
Details on test organisms:
The test was performed with Gobiocypris rarus which were obtained from the Institute of Hydrobiology, Chinese Academy of Sciences (ID: GR-SAPM-180112). This freshwater fish species is suitable for fish toxicity testing because of their ease of culturing and handling, sensitivity to a variety of chemical substances, and the extensive data base for this species. This species complies with Ministry of environmental protection of the people's Republic of China, The Guidelines for the Testing of Chemicals-Effects on Biotic System, 2013, 203 Fish, Acute Toxicity Test, OECD, Guidelines for the Testing of Chemicals. 203 Fish, Acute Toxicity Test, 1992
and GB/T 29763-2013 Chemicals-Rare Minnow (Gobiocypris rarus) acute toxicity test.

For evaluation of the fish quality and experimental conditions, potassium dichromate was tested as a positive control to demonstrate satisfactory test conditions once a year(recently performed on 2015.7.7-7.11). The 96h LCso value of the test substance to Gobiocypris rarus was 257mg/L(95% confidence limits: 207-309mg/L), which was within the ± 2SD range of the QC chart in our lab(82.2-309mg/L).

Photoperiod: Light/Dark= 12 hours/12 hours;
Feeding: fish were fed with brine shrimp flake (O.S.I®, USA) daily until 24 hours before the test was started.
Length was 2.4 ± 0.1 cm (Mean ± SD) and weight was 0.21±0.01 g (Mean ± SD) at the end of the test.
No diseases were observed during the acclimation period;
No mortality was present within the 7 days before the start of the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Remarks on exposure duration:
renewal every 24 h
Test temperature:
21 - 25 °C
pH:
6 to 8.5
Dissolved oxygen:
> 80 %
Nominal and measured concentrations:
loading rate of 100 mg/L (mean measured concentration < LOD2, LOD2=52.4 ng/mL)
Details on test conditions:
none
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
96 h
Dose descriptor:
LC50
Remarks:
ng/mL
Effect conc.:
> 52.4 other:
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
not determinable
Remarks:
LC 50 was above solubility limit
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
> 52.4 other: ng/mL
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: solubility limit in test solution
Details on results:
The test results showed that the 96h LC50 for the test substance to Gobiocypris rarus was higher than the concentration of the saturated solution under tested conditions. During the lest, the measured concentrations of the lest substance were below the limit of detection {LOD2, LOD2=52.4ng/ml). There were no mortalities and abnormalities observed in the fish of the control group and the treatment group during the lest.
Results with reference substance (positive control):
For evaluation of the fish quality and experimental conditions, potassium dichromate was tested as a positive control to demonstrate satisfactory test conditions once a year(recently performed on 20170501). The 96h LC50 value of the test substance to Gobiocypris rarus was 257mg/L(95% confidence limits: 207-309mg/L), which was within the ± 2SD range of the QC chart in our lab(82.2-310mg/L).
Validity criteria fulfilled:
yes
Conclusions:
The 96h LC50 of the lest substance to Gobiocypris rarus was higher than the concentration of the saturated solution with a loading rate of 100 mg/L (the measured concentration was below the LOD2, LOD2=52.4ng/mL) under the tested conditions.

Description of key information

The 96h LC50 of the lest substance to Gobiocypris rarus was higher than the concentration of the saturated solution with a loading rate of 100 mg/L (the measured concentration was below the LOD2, LOD2=52.4ng/mL) under the tested conditions.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information