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EC number: 949-790-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 February 2019 - 04 April 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- adopted July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: SO International Standard 10634. "Water Quality - Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium"
- Version / remarks:
- 1995
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Identification: Esacure 3644
Physical Description: Light yellow solid
Purity/Composition: UVCB
Storage Conditions: At room temperature protected from light - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- SOURCE
The source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
TREATMENT
Freshly obtained sludge was kept under continuous aeration until further treatment. Before use, sludge was coarsely sieved (1 mm). After treatment concentration of suspended solids (SS) was determined to be 3.4 g/L in concentrated
sludge as used for the test. Magnetically stirred sludge was used as inoculum at an amount of 3 mL per litre of mineral medium, leading to a SS concentration of 10 mg/L.
REASON FOR SELECTION
The test has been accepted internationally for determining 'ready' biodegradability of test items under aerobic conditions. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 17 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST PROCEDURE AND CONDITIONS
TEST DURATION
28 days for inoculum blank and test item (last CO2 measurement on day 29).
14 days for procedure and toxicity control (last CO2 measurement on day 15).
During the test period, test media were aerated and stirred continuously.
TEST VESSELS
2 litre brown coloured glass bottles.
MILLI- RO WATER
Tap-water purified by reverse osmosis (Milli- RO) and subsequently passed over activated carbon.
STOCK SOLUTIONS OF MINERAL COMPONENTS
(A) 8.50 g KH2PO4
21.75 g K2HPO4
67.20 g Na2HPO4.12H2O
0.50 g NH4Cl
dissolved in Milli- RO water and made up to 1 litre, pH 7.4 ± 0.2
(B) 22.50 g MgSO4.7H2O dissolved in Milli- RO water and made up to 1 litre.
(C) 36.40 g CaCl2.2H2O dissolved in Milli- RO water and made up to 1 litre.
(D) 0.25 g FeCl3.6H2O dissolved in Milli- RO water and made up to 1 litre.
MINERAL MEDIUM
1 litre mineral medium contains: 10 mL of solution (A), 1 mL of solutions (B) to (D) and Milli- RO water.
BARIUM HYDROXIDE
0.0125 M Ba(OH)2 (Boom, Meppel, The Netherlands), stored in a sealed vessel to prevent absorption of CO2 from air.
SYNTHETIC AIR (CO2 < 1 PPM)
A mixture of oxygen (ca. 20%) and nitrogen (ca. 80%) was passed through a bottle, containing 0.5 - 1 litre 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. Synthetic air was passed through the scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
ILLUMINATION
Test media were excluded from light.
SAMPLING AND CONTROLS
Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for inoculum blank and test item. Titrations for procedure and toxicity control were made over a period of at least 14 days.
Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers were moved one position in the direction of the test bottle.
A new CO2-absorber was placed at the far end of the series.
Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
On the penultimate day, pH of respective test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the inoculum blank and test suspension.
Bottles were aerated overnight to drive off CO2 present in the test suspension. Final titration was made on day 15 (procedure and toxicity control) and on day 29 (remaining vessels).
The study consisted of six bottles:
2 inoculum blanks (no test item),
2 test bottles (Esacure 3644),
1 procedure control (sodium acetate) and
1 toxicity control (Esacure 3644 plus sodium acetate). - Reference substance:
- acetic acid, sodium salt
- Test performance:
- Since all criteria for acceptability of the test were met, this study was considered to be valid.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Since Esacure 3644 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to 2 litre test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli- RO water was added to each weighing bottle containing test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. Test solutions were continuously stirred during the test to ensure optimal contact between test item and test organisms. Furthermore, test medium was swirled around daily, since the test item tended to float on the water surface. Test duration was 28 days for inoculum blank and test item (last CO2 measurement on day 29) and 14 days for procedure and toxicity control (last CO2 measurement on day 15).
Relative biodegradation values calculated from measurements performed during the test period revealed no biologically relevant biodegradation of Esacure 3644 (2% and 0%, based on ThCO2).
In the toxicity control, Esacure 3644 was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, Esacure 3644 was designated as not readily biodegradable. - Results with reference substance:
- Functioning of the test system was checked by testing the reference item sodium acetate, which showed a normal biodegradation curve
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In conclusion, Esacure 3644 was not readily biodegradable under the conditions of the modified Sturm test presently performed.
- Executive summary:
The objective of the study was to evaluate test item Esacure 3644 for its ready biodegradability in an aerobic aqueous medium with microbial activity introduced by inoculation with activated sludge.
Procedures described in this report were in compliance with OECD guideline No. 301 B, 1992. In addition, procedures were designed to meet test methods of ISO standard 10634, 1995.
Esacure 3644 was a light yellow solid (UVCB) and was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L. Total Organic Carbon (TOC) content of the test item was determined to be 71.54%. Based on TOC content ThCO2 of the test item was calculated to be 2.62 mg CO2/mg. The test item was tested in duplicate at a target concentration of 17 mg/L, corresponding to 12 mg TOC/L.
The study consisted of six bottles:
2 inoculum blanks (no test item),
2 test bottles (Esacure 3644),
1 procedure control (sodium acetate) and
1 toxicity control (Esacure 3644 plus sodium acetate).
Since Esacure 3644 was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/L, weighed amounts were added to 2 litre test bottles containing medium with microbial organisms and mineral components. To this end, 10 mL of Milli- RO water was added to each weighing bottle containing test item. After vigorous mixing (vortex) the resulting suspension was added quantitatively to the test medium. Test solutions were continuously stirred during the test to ensure optimal contact between test item and test organisms. Furthermore, test medium was swirled around daily, since the test item tended to float on the water surface. Test duration was 28 days for inoculum blank and test item (last CO2 measurement on day 29) and 14 days for procedure and toxicity control (last CO2 measurement on day 15).
Relative biodegradation values calculated from measurements performed during the test period revealed no biologically relevant biodegradation of Esacure 3644 (2% and 0%, based on ThCO2).
In the toxicity control, Esacure 3644 was found not to inhibit microbial activity.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, Esacure 3644 was designated as not readily biodegradable.
Reference
For full results see appendix 1 attached to background information
Description of key information
Study conducted to recognised testing guidelines with GLP certification
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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