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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
not specified
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.14 g suspended solids/L, giving a concentration of 1.07 g suspended solids/L in the test.
Test type:
not specified
Limit test:
yes
Total exposure duration:
3 h
Hardness:
tap water
Test temperature:
22. 1 – 24.7 °C (first experiment (pre-test))
21.7 -24.8 °C (second experiment (main test))
pH:
7.6-7.9
Reference substance (positive control):
yes
Remarks:
1 replicate/concentration (all experiments)
Key result
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
ca. 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
ca. 630 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Key result
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
ca. 3 700 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Validity criteria fulfilled:
yes
Conclusions:
3h NOEC = 320 mg/L
3h EC10 = 630 mg/L (confidence interval: 420 – 810 mg/L)
3h EC50 = 3700 mg/L (extrapolated) confidence interval: not determinable

Description of key information

3h NOEC = 320 mg/L

3h EC10 = 630 mg/L (confidence interval: 420 – 810 mg/L)

3h EC50 = 3700 mg/L (extrapolated) confidence interval: not determinable

Key value for chemical safety assessment

EC50 for microorganisms:
3 700 mg/L
EC10 or NOEC for microorganisms:
320 mg/L

Additional information