4-amino-2-methylpyrimidine-5-carbonitrile

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06/1982 - 07/1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: good test description available, no GLP oder OECD guidlines followed

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

not specified

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

4-amino-2-methylpyrimidine-5-carbonitrile

EC Number:

211-814-2

EC Name:

4-amino-2-methylpyrimidine-5-carbonitrile

Cas Number:

698-29-3

Molecular formula:

C6H6N4

IUPAC Name:

4-amino-2-methylpyrimidine-5-carbonitrile

Method

Target gene:

all 5 strains contain the deep rough (rfa) mutation, which deletes the polysaccharide side chain of the lipopolysaccharide coat of the bacterial cell surface

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Additional strain / cell type characteristics:

other: mutations in the histidine operon. TA 1538 was used intead of TA 1535

Metabolic activation:

with and without

Metabolic activation system:

mixed function oxidase system

Test concentrations with justification for top dose:

5, 50, 500 and 5000 ug/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

yes

Positive controls:

yes

Positive control substance:

2-nitrofluorene

Remarks:

Migrated to IUCLID6: 9-aminoacridine, N-ethyl-N-nitro-N-nitroso-guanidine, 2-aminoanthracene, benzo-(a)pyrene, 2- (2-furyl)-3- (5- nitro-2-fury1 acrylamide

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

not determined

Cytotoxicity / choice of top concentrations:

not determined

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

it is concluded that Pynitril itself nor its metabolites produced by rat liver enzymes are mutagenic under the conditions emploid.

Executive summary:

the Ames test with Pynitril was performed in 1980 according to the (at that time) state of the art practices. None of the revertant colonies increased over the back ground (negative control) in the dose range from 5 to 5000 pg/plate with or without metabolic activation by a rat liver homogenate (S-9 Mix.).

On the other hand, positive control compounds were clearly mutagenic under the conditions employed