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EC number: 807-577-5
CAS number: 1182844-21-8
The objective of this study was to determine the influence of the test
item on the growth and growth rate of the unicellular green algal
species Desmodesmus subspicatus. The study design included one control
group and one test material group with six replicates, each containing
100 mL reconstituted water or test medium and about 10000 cells/mL at
the start of the experimental phase.
The study was performed as a limit test in an open static test system.
The algae were exposed to s filtrate of nominal 100 mg test material per
L. The growth of the algae was calculated after 24, 48, and 72 hours
exposure in the test item and control groups.
This study was performed according to GLP and the methods applied are
fully compliant with OECD TG 201.
The test material concentration in the reconstituted water was not
quantified at the start and the end of this study due to the low water
solubility (< 0.051 mg/L). Because of the low water solubility, the
compound cannot be detected with standard analytical methods. The
development of an analytical method with a sufficiently low detection
and quantification limit is complex. Due to the absence of any
adverse effects at the saturation concentration, the study was
performed without analytical concentration verification.
For the test item, the following values for Desmodesmus
subspicatus were determined:
aqueous preparation of 100 mg/L (nominal) of the test item revealed no
toxic effect in the test system. The 72 hours EC50 and EC10 could not be
determined, because it exceeded the maximum solubility of the test
material in reconstituted water (EC50 > 0.051 mg/L).
No adverse effects on algae were observed up to the limit of water
solubility. Thus, the 72 -h EC50 value and 72 -h EC10 value are greater
than 0.051 mg/L which corresponds to a nominal concentration of 100 mg/L.
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