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EC number: 821-451-7
CAS number: 78920-10-2
Table 1: CO2 Production and
Percentage Biodegradation of the Test Item (Bottle A)
HCl (0.05 N) titrated (mL)
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 88.2 mg CO2/2L.
2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl.
Table 2: CO2 Production and
Percentage Biodegradation of the Test Item (Bottle B)
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 88.0 mg CO2/2L.
In a CO2 Evolution Test was performed
according to OECD guideline 301B and GLP principles, the substance was
evaluated for its ready biodegrabaility. The test was performed in an
aerobic aqueous medium containing activated sludge over a period of 28
Due to a purity <95%, the substance's Total
Organic Carbon (TOC) content was determined by TOC analysis. The TOC
content was 58.33% and this value was used to calculate the ThCO2 of the
substance (2.14 mg CO2/mg).
The substance was tested in duplicate at a
target concentration of 20.5 mg/L, corresponding to 12 mg TOC/L. Two
inoculum blanks, one procedure control (sodium acetate) and one toxicity
control (substance plus sodium acetate) were included. During the 28
days exposure period, the produced CO2 was determined every second or
third day for the first 10 days and at least every fifth day from day 11
to day 28. Relative biodegradation values were calculated from the CO2
production relative to the ThCO2.
The substance biodegraded for 67% and 61% in
duplicate bottles tested. However, as the substance did not reach ≥60%
biodegradation within a 10 -day window, the criterion for ready
biodegradability was not met. Since 46% biodegradation occurred in the
toxicity control within 14 days, the substance was assumed not to
inhibit microbial acitivity.
All set acceptability criteria for the test
were met and the study is therefore considered to be valid.
Based on the obtained results in this test,
the substance is concluded to be not readily biodegrable.
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