reaction mass of sodium 1-amino-4-[[3,5-bis[[(chloroacetyl)amino]methyl]-2,4,6-trimethylphenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate and sodium 1-amino-4-[[3-[[(chloroacetyl)amino]methyl]-2,4,6-trimethylphenyl]amino]-9,10-dihydro-9,10-dioxoanthracene-2-sulphonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From August 30 to September 18, 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Water solubility:

12.87 g/L

Conc. based on:

test mat.

Incubation duration:

24 - 72 h

Temp.:

20 °C

pH:

2.4

Details on results:

For the main test three flasks were prepared with an amount of test item and water and one flask only with water. After stirring and centrifugation the aqueous phases were measured by means of HPLC. The solution of each flask has been measured twice at 20 °C.
To verify the accuracy of the analytical method two fresh prepared standard solutions (prepared with the test item) were measured in between the samples of the flask method. The recovery rate of the measured standard solutions was in the range from 98.3 to 103.0 %, which shows a good accuracy of the analytical method.

PRELIMINARY TEST
The water solubility of the test item was between 28.4 and 63.8 g/l.

Flask	1a	2a	3a	4a
measured value [mg/l]	123.84 / 121.75	125.79 / 126.67	134.16 / 140.06	-- / --
average water solubility [g/l]*	12.28	12.62	13.71	--
mean value of the water solubility [g/l]	12.87			--
standard deviation [g/l]	0.75			--
relative standard deviation [%]	5.8			--

*Under consideration of the dilution factor 100

Conclusions:

12.87 g/l at 20 °C and pH 2.4

Executive summary:

The water solubility of the test item was determined in accordante with the method and procedures outlined into the OECD guideline 105.

A preliminary test for solubility was carried out for the decision whether the column elution method or the flask method has to be used for the performance of the main test. The solubility of the test item was higher than 10 mg/l. Therefore, the flask method was used for the main test.

The concentration in the clear aqueous phase was determined by HPLC-UV-Vis validate method. The pH of each sample was recorded.

For the main test three flasks were prepared with an amount of test item and water and one flask only with water. After stirring and centrifugation the aqueous phases were measured by means of HPLC. The solution of each flask has been measured twice at 20 °C.

To verify the accuracy of the analytical method two fresh prepared standard solutions (prepared with the test item) were measured in between the samples of the flask method. The recovery rate of the measured standard solutions was in the range from 98.3 to 103.0 %, which shows a good accuracy of the analytical method.

The water solubility of the test item resulted to be 12.87 g/l at 20 °C and pH 2.4.

Conclusion

Water solubility: 12.87 g/l at 20 °C and pH 2.4.

Description of key information

12.87 g/l at 20 °C and pH 2.4

Key value for chemical safety assessment

Water solubility:

12.87 g/L

at the temperature of:

20 °C

Additional information

OECD 105 - Flask method