Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019
Report date:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
GLP compliance:
yes (incl. QA statement)
Type of method:
column elution method

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
EC Number:
300-634-0
EC Name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
Cas Number:
93951-12-3
Molecular formula:
C28H39Cl2NO3
IUPAC Name:
2-[2,4-bis(tert-pentyl)phenoxy]-N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)butyramide
Test material form:
solid
Details on test material:
Batch 44029
colour : White to off-white
retest date : 01/06/2019
Specific details on test material used for the study:
Storage conditions:
Room temperature (15-25°C, <= 70 RH%);
Protected from humidity (tight closed container)
Protected from light

Results and discussion

Water solubility
Key result
Water solubility:
153.4 µg/L
Conc. based on:
test mat.
Incubation duration:
2 h
Temp.:
20 °C
pH:
6
Details on results:
DEVIATION FROM THE STUDY PLAN:
Because of the better solubility, the test item was dissolved in tetrahydrofuran instead of acetone. The deviation has no impact on the integrity of the study.

Any other information on results incl. tables

The validated analytical method (FPBSTUDY-196-VAL1-REPORT-01; Citoxlab study code: 18/269-901AN) was used for the determination of the test item content in aqueous solutions

Results of the Method Validation:

 Specificity

 Interfering components conform to less than 20% of the LOQ criteria

 

 

 Repeatability (7 injections)

 3.97 %

 

 

 Dynamic range

 0.019 – 0.60 ng/mL

 

 

 Limit of Quantification

0.019 ng/mL analytical sample concentration

 

 

0.019 μg/L sample concentration

 Recovery and Precision

 

 

 

 

 Concentration (μg/L)

 100

 0.04

 Recovery (%)

 95.3

 99.5

 Precision (%)

 3.61

 3.82

 Stability of the analytical sample (%)

 

 

 

 

 9 h 29 min  (%)

100.8

 

 

Applicant's summary and conclusion

Conclusions:
The solubility of the test item in HPLC water at 20 ± 0.5°C is 153.4±5.72 μg/L.
Executive summary:

The first two days the measured values increased continuously. The static concentrations were reached after the 4th sampling point.

The deviation among the last 5 parallel results of the columns were less than 30% and random fashioned so all the 10 values were averaged, and the standard deviation calculated. The solubility is given as mean±SD.