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EC number: 466-080-8 | CAS number: -
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 October 2006 to 27 October 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Version / remarks:
- No. L383/A, 29.12.92
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Version / remarks:
- Paris, 1981
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test article identification : LFC 2098
Chemical name : Bis N,N—[2-(4-dimethylaminobenzoyl)oxyethylen-1-yl]methylamine
Chemical formula: C23H31O4N3
Molecular weight : 413
Chemical Class : Aminoester
Declared purity : 100.0 %
Preparation date : May 29, 2006
Sample was stored at room temperature in the dark - Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Preliminary test: Samples at 0 h, 2.5 h and 5 days
Full test: Samples at 0, 16, 24, 40, 48, 64, 72, and 88 h - Buffers:
- pH 4
30 mL of H3PO4 0.1 N were added to 100 mL of KH2P04 0.1 N (1.36 g of KH2P04 in 100 mL of distilled water), then diluted to 200 mL with distilled water.
pH 7
59 mL of NaOH 0.1 N were added to 100 mL of KH2P04 0.1 N (1.36 g of KHZPO4 in 100 mL of distilled water), then diluted to 200 mL with distilled water.
pH 9
42 mL of NaOH 0.1 N were added to 100 mL of 0.1 M H3803 (0.63 g of H3BO3 in 100 mL of distilled water), then diluted to 200 mL with distilled water. - Details on test conditions:
- A preliminary test is performed at 50 ± 0.5°C at three pH values, 4.0 ; 7.0 and 9.0.
If this test indicates that 50% or more of the test article has been hydrolysed in 2.5 hours at 50°C, or less than 10% has been hydrolysed after five days at each of the three pH values (4.0 ; 7.0 and 9.0), no further testing is necessary.
The experiment is carried out at 50 ± 0.5°C. and several samples of each pH solution are analysed at different times in order to cover a suitable range of hydrolysis to test for pseudo- first order behaviour of the reaction.
From the calculation of the data obtained by this test, it is reliably possible to conclude if the hydrolysis reaction is pseudo-first order, then the test is repeated for each pH value either at one temperature lower than 40°C or at two temperatures above 50°C, differing from each other by at least 10°C. - Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Remarks:
- Preliminary
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Remarks:
- Preliminary
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Remarks:
- Preliminary
- Duration:
- 88 h
- pH:
- 7
- Temp.:
- 50 °C
- Remarks:
- Full test
- Duration:
- 88 h
- pH:
- 9
- Temp.:
- 50 °C
- Remarks:
- Full test
- Number of replicates:
- 2 replicates for each pH
- Positive controls:
- no
- Negative controls:
- no
- Statistical methods:
- Linear regression analyses were performed, using the least squares method, reporting the time versus logn of the percentage of degradation and calculating the kobs values from the slope of the linear curves obtained and consequently, the t1/2 values.
- Preliminary study:
- The results of the preliminary test showed that less than 50% of the test article at three pH values (4.0 ; 7.0 and 9.0) has been hydrolysed in 2.5 hours at 50°C, but more than 10% of the test substance at pH 7.0 and pH 9.0 has been hydrolysed after five days.
Therefore, according to the guideline in Official Journal L 283 0.7, the full test must be conducted for pH 7.0 and pH 9.0. - Transformation products:
- not measured
- pH:
- 7
- Temp.:
- 50 °C
- DT50:
- 109 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 50 °C
- DT50:
- 100 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 50 °C
- DT50:
- 57 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 50 °C
- DT50:
- 54 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 40 °C
- DT50:
- 276 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 40 °C
- DT50:
- 277 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 40 °C
- DT50:
- 194 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 40 °C
- DT50:
- 198 h
- Type:
- (pseudo-)first order (= half-life)
- Details on results:
- pH 7.0, 50 °C mean: 104 h
pH 9.0, 50 °C mean: 56 h
pH 7.0, 40 °C mean: 276 h
pH 9.0, 40 °C mean: 196 h - Validity criteria fulfilled:
- yes
- Conclusions:
- From the experimental data obtained it can be concluded that the abiotic degradation hydrolysis of LFC 2098 sample is lower than 10% after 5 days at 50°C for the pH 4.0, but is significant at pH 7 and pH 9 ( > 10% in 5 days at 50°C in the preliminary test).
The full test was then performed at 50°C and 40°C for the pH 7 and pH 9, determining the kobs rate constant and Mg values for these temperatures.
From these experimental data, the values at 25°C were calculated by extrapolation from the empirical Arrhenius equation. - Executive summary:
pH value Temperature (°C) Slope kobs (h-1) t1/2 (h) From the experimental data 7 50 0.0067 104 7 40 0.0025 276 From the calculation with Arrhenius equation 7 25 0.0005 1357 From the experimental data 9 50 0.0125 56 9 40 0.0035 196 From the calculation with Arrhenius equation 9 25 0.0005 1 522
Reference
pH 7.0, 50 °C
| Sampling Code | Correlation coefficient R | Intercept | Slope kobs(h-1) | t1/2 (h) |
| pH 7 A | 0.99978 | -0.0034 | 0.0064 | 109 |
| pH 7 B | 0.99937 | -0.0047 | 0.007 | 100 |
| Mean value : | 0.0067 | 104 |
||
pH 9.0, 50 °C
| Sampling Code | Correlation coefficient R | Intercept | Slope kobs(h-1) | t1/2 (h) |
| pH 9 A | 0.99961 | -0.0105 | 0.0121 | 57 |
| pH 9 B | 0.99908 | -0.0134 | 0.0128 | 54 |
| Mean value : | 0.0125 | 56 | ||
pH 7.0, 40 °C
| Sampling Code | Correlation coefficient R | Intercept | Slope kobs(h-1) | t1/2 (h) |
| pH 7 A | 0.98987 | 0.0024 | 0.0025 | 276 |
| pH 7 B | 0.98989 | -0.0026 | 0.0025 | 277 |
| Mean value : | 0.0025 | 276 | ||
pH 9.0, 40 °C mean
| Sampling Code | Correlation coefficient R | Intercept | Slope kobs(h-1) | t1/2 (h) |
| pH 9 A | 0.99714 | -0.0052 | 0.0036 | 194 |
| pH 9 B | 0.99368 | 0.0038 | 0.0035 | 198 |
| Mean value : | 0.0035 | 196 | ||
Description of key information
Study conducted to recognised testing guidelines with GLP certification.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 522 h
- at the temperature of:
- 25 °C
Additional information
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