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EC number: 947-964-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted July 17, 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 31 May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Version / remarks:
- October 2016
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Amount of total organic carbon (TOC) was determined in the highest test concentration and the control at the start and at the end of each water renewal period. The test concentration of 54.4 mg/L was analysed as highest concentration at the 4th renewal period as all fish died in the 100.0 mg/L concentration by that period. Three replicates were analysed from the test concentration and the control respectively at each analytical occasion.
- Vehicle:
- no
- Details on test solutions:
- The test item is a poorly water soluble UVCB material therefore a water-accommodated fraction (WAF) was prepared as follows: a supersaturated solution (100 mg/L nominal loading) was prepared by dispersing/dissolving the test item amount into the test medium (ISO medium) in a sealed Erlenmeyer flask filled up fully, thus allowing no headspace. This solution was stirred for 24 hours using magnetic stirrer and then settle for approx. one hour to allow phase separation. The saturated solution was removed from the approximate middle of flask. The test solutions of subsequent lower test concentrations was prepared by appropriate dilution of this stock WAF.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: Akvárium magazin Kft. (Pasaréti Gyula) 1222 Budapest, Dévény u. 36.
- Length at study initiation: 1.55 - 2.35 cm
- Weight at study initiation: 0.09 - 0.11 g
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions: same as test conditions
- Type and amount of food during acclimation: appropriate, commercial diet for fish
- Feeding frequency during acclimation: at least three times per week until one day before the test start
- Health during acclimation: no mortality observed
FEEDING DURING TEST
The fish will not be fed during the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 249 mg/L (as CaCO3).
- Test temperature:
- 21.4 – 22.1°C
- pH:
- 7.36 – 8.01
- Dissolved oxygen:
- 65.1 – 89.2 %
- Nominal and measured concentrations:
- Nominal concentrations: 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L (loading rate), no measured concentrations
The nominal concentrations were recalculated based on the water solubility of 17.817 mg/L (see IUCLID section 4.8). The calculated concentrations were: 0.76, 1.67, 3.69, 8.11 and 17.8 mg/L. - Details on test conditions:
- TEST SYSTEM
- Test vessel: aquarium
- Type: closed (sealed by glass plates)
- Material, headspace, fill volume: glass, no headspace, 2300mL
- Aeration: no
- Renewal rate of test solution: 24-h water renewal periods
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: reconstituted water (ISO medium, prepared according to recommendation of Annex 2 of the OECD 203 guideline
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light: 8 h dark
EFFECT PARAMETERS MEASURED
mortality at approximately 24, 48, 72 and 96 hours after start of the test
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: yes, LC50 between 10 and 100 mg/L - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: recalculated based on the water solubility
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: recalculated based on the water solubility
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 8.11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: recalculated based on the water solubility
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- 58.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: based on nominal loading rate
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 20.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: based on nominal loading rate
- Duration:
- 96 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 45.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: based on nominal loading rate
- Details on results:
- No mortality was observed in the control during the test. The dissolved oxygen concentration in the test solutions did not fall below 60 % of air saturation value during the study. No significant change (more than one unit) in the pH value was observed during the test. All validity criteria were within acceptable limits and therefore the study was considered as valid.
- Reported statistics and error estimates:
- For determination of the LC50 value Probit analysis was performed using SPSS software. Other endpoints were determined directly from the raw data.
- Sublethal observations / clinical signs:
Table 1: Cumulative mortality data
Concentration
[mg/L loading rate]Cumulative mortality
(initial population = 7 fish / group)4h
24h
48h
53h
72h
73h
96h
Control
0
0
0
0
0
0
0
4.3
0
0
0
0
0
0
0
9.4
0
0
0
0
0
0
0
20.7
0
0
0
0
0
0
0
45.5
0
0
0
0
0
0
1
100.0
0
2
5
7
7
7
7
Table 2: Sub-lethal effects observed during the study
Concentration
[mg/L loading rate]Symptoms
Numbers of fish / Observation time
4h
24h
48h
53h*
72h
73h#
96h
Control
–
–
–
–
–
–
Cumulative mortality
0
0
0
0
0
0
0
4.3
–
–
–
–
–
–
Cumulative mortality
0
0
0
0
0
0
0
9.4
–
–
–
–
–
–
Cumulative mortality
0
0
0
0
0
0
0
20.7
–
–
–
–
–
–
Cumulative mortality
0
0
0
0
0
0
0
45.5
Slightly reduced activity (observed only after water renewal, in fresh test solution)
7
7
7
7
0
0
Slightly reduced activity (observed in old test solution, before water renewal as well)
0
0
0
0
0
0
Reduced activity
0
0
4
5
3
6
Lying at the bottom of aquarium
0
0
0
0
4
0
Cumulative mortality
0
0
0
0
0
0
1
100.0
Severely reduced activity; off-balance; swimming only periodically; lying at the bottom of aquarium;
7
4
2
Lying at the bottom of aquarium; gill movements only
0
1
0
Cumulative mortality
0
2
5
7
7
7
7
– no sign of symptoms
* 53-h observation period was included only in concentration of 100 mg/L (loading rate)
# 73-h observation period was included only in concentration of 45.5 mg/L (loading rate)
Table 3: Total organic carbon (TOC) concentrations measured during the test
Nominal concentration
[mg/L loading rate]Measured TOCconcentration[mg/L]
1st renewal period
2nd renewal period
3rd renewal period
4th renewal period
start
end
start
end
start
end
start
end
100.0
4.49
3.32
4.03
4.19
3.50*
3.16
–
–
45.5
–
–
–
–
1.44*
0.57*
* these values were below the quantification limit (< BQL) based on the actual daily calibration curves
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a semi-static short term study the test item was found to affect the mortality of Danio rerio after 96 hours with the following effect values: The EC50 value was 10.4 mg/L, recalculated based on the water solubility of the test item.
- Executive summary:
The purpose of this study according to OECD guideline 203 was to evaluate the influence of the test item on zebrafish (Danio rerio). Young fish were exposed in an acute toxicity test to aqueous test media containing the test item for 96 hours at a range of concentration in a semi-static test system (daily renewal periods). The test method of application and the test species Zebrafish (Danio rerio) are recommended by the test guidelines. Based on the results of the preliminary experiment, nominal concentrations of 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L (loading rate) were investigated in the main study. Biological results are based on the nominal loading rates. Seven fish were exposed to each concentration and a control for 96 hours. The fish were observed at least on each day of the test for signs of intoxication and mortality. Body weights of fish were determined prior to test initiation; body length measurement was performed at the end of the test (or when any mortality occurred). Measurements of environmental conditions were performed daily (at the start and end of each water renewal period). Sealed glass aquariums filled up fully with the test solution (thus allowing no headspace) were used as test vessels. The volume of the test liquid in the vessels was approx. 2300 mL. Amount of total organic carbon (TOC) was determined in the highest test concentration and the control at the start and at the end of each water renewal period immediately after sampling. The endpoints used in this study are LL50, NOELR and LOELR for 96-h test period. For determination of the LL50 value Probit analysis was performed using SPSS software. Other endpoints were determined directly from the raw data. All validity criteria were met and therefore the study can be considered as valid. The measured mean total organic carbon (TOC) concentration was 4.49 mg/L at the start and 0.57 mg/L (below the quantification limit) at the end of the test. In this 96-hour semi-static acute toxicity test on Zebrafish (Danio rerio) the test item had significant toxic effects on fish. The 96-h LL50 value was determined to be 58.4 mg/L (loading rate). The NOELR was determined to be 20.7 mg/L (loading rate) and the LOELR was determined to be 45.5 mg/L (loading rate). All results were recalculated based on the water solubility of the test item, the LC50 was determined to be 10.4 mg/L, the NOEC 3.69 mg/L and the LOEC 8.11 mg/L.
Reference
Description of key information
In a semi-static short term study the test item was found to affect the mortality of Danio rerio after 96 hours with the following effect values: The EC50 value was 10.4 mg/L, recalculated based on the water solubility of the test item.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10.4 mg/L
Additional information
The purpose of this study according to OECD guideline 203 was to evaluate the influence of the test item on zebrafish (Danio rerio). Young fish were exposed in an acute toxicity test to aqueous test media containing the test item for 96 hours at a range of concentration in a semi-static test system (daily renewal periods). The test method of application and the test species Zebrafish (Danio rerio) are recommended by the test guidelines. Based on the results of the preliminary experiment, nominal concentrations of 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L (loading rate) were investigated in the main study. Biological results are based on the nominal loading rates. Seven fish were exposed to each concentration and a control for 96 hours. The fish were observed at least on each day of the test for signs of intoxication and mortality. Body weights of fish were determined prior to test initiation; body length measurement was performed at the end of the test (or when any mortality occurred). Measurements of environmental conditions were performed daily (at the start and end of each water renewal period). Sealed glass aquariums filled up fully with the test solution (thus allowing no headspace) were used as test vessels. The volume of the test liquid in the vessels was approx. 2300 mL. Amount of total organic carbon (TOC) was determined in the highest test concentration and the control at the start and at the end of each water renewal period immediately after sampling. The endpoints used in this study are LL50, NOELR and LOELR for 96-h test period. For determination of the LL50 value Probit analysis was performed using SPSS software. Other endpoints were determined directly from the raw data. All validity criteria were met and therefore the study can be considered as valid. The measured mean total organic carbon (TOC) concentration was 4.49 mg/L at the start and 0.57 mg/L (below the quantification limit) at the end of the test. In this 96-hour semi-static acute toxicity test on Zebrafish (Danio rerio) the test item had significant toxic effects on fish. The 96-h LL50 value was determined to be 58.4 mg/L (loading rate). The NOELR was determined to be 20.7 mg/L (loading rate) and the LOELR was determined to be 45.5 mg/L (loading rate). All results were recalculated based on the water solubility of the test item, the LC50 was determined to be 10.4 mg/L, the NOEC 3.69 mg/L and the LOEC 8.11 mg/L.
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