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EC number: 600-896-9 | CAS number: 109089-77-2
Table 2. Summary of results.
> 100 mg/L
Table 3. Daphnids immobilisation results.
Nominal test item concentration( mg/L)
Number of daphnids
exposed at the start
Immobilised daphnids (%)
Table 4. Measured test item concentration during the main test (variation < 20% of the nominal value).
Concentration (mg test item / L)
Nominal test item concentration
Initial time (T0h)
Final time (T48h)
Table 5. Validity criteria.
Values for OECD 202
Validity criteria for the test
Immobilised daphnids in control
Dissolved Oxygen concentration
> 3 mg/L
Ok (8.5 mg/L)
Table 6. pH values
Table 7. Dissolved oxygen.
(Air: 9.4 mg/L)
An acute immobilisation test on Daphnia magna was performed on the test item, according to OECD 202, under GLP conditions. Based on the results obtained in a preliminary test where immobilisation of 20 - 40% was observed at 328 - 1050 mg/L test item, a 48-h exposure limit test at 100 mg/L test item concentration was performed. Blank controls were run in parallel, and a positive control is regularly performed with potassium dichromate. Six replicates were tested for the control and the test item. Test concentrations were analytically determined by means of a validated HPLC-UV method at the beginning and at the end of the test. Since the test item was stable in the test medium (deviation from the measured initial concentration was within ± 20 %), the test results expressed relative to nominal test item concentrations. All validity criteria were met. The 48h-EC50 and the 24h-EC50 of the test item in daphnids were determined to be > 100 mg/L.
ECOSAR v1.11 predicted that the substance has a 48h-LC50 = 528.7 mg/L in daphnia.
According to the prediction, if the effect level exceeds the water solubility by 10X, typically no effects at saturation (NES) are predicted. The experimental water solubility value of the test item was found to be 14.2 mg/L (20ºC, pH 5).
ECOSAR v1.11 calculation of the 48h-LC50 in daphnia was used. According to this method, the 96h-LC50 = 528.7 mg/L of test item in daphnia.
Key study. Method according to OECD 202, GLP study. The 48h-EC50 and the 24h-EC50 of the test item in daphnids were determined to be > 100 mg/L.
Supporting study. ECOSAR v1.11, accepted calculation method. The substance has a predicted 48h-LC50 = 528.7 mg/L in daphnia.
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