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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From August 31st, 2018 to September 7th, 2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
flask method
Key result
Water solubility:
0.014 g/L
Conc. based on:
test mat. (total fraction)
Incubation duration:
72 h
Temp.:
20 °C
pH:
>= 4.99 - <= 5.09

Table 1. Results of the test item solubility in water

Mixing time at 30 ºC

[days, flasks]

1

2

3

Samples

1

2

3

4

5

6

Temperature, ºC

20.1

20.0

20.2

pH

5.09

5.06

4.99

Concentration of the test item, g/kg

 

0.0143

 

0.0141

 

0.0143

 

0.0144

 

0.0139

 

0.0141

Average concentration of the test item, g/kg

 

0.0142

 

0.0144

 

0.0140

Average concentration the test item, g/kg

 

0.0142

 

Table 2. Results of the preliminary test

0.1g in x mL of solution

0.1

0.5

1

2

10

100

> 100

Soluble (+/-)

-

-

-

-

-

-

+

Approximate solubility, g/L

> 1000

1000 to

200

200 to 100

100 to 50

50 to 10

10 to 1

< 1

 

Table 3. Calibration solution

 

Day

Weight [mg]

Dilution [mL]

Cw [mg/mL]

 

Peak area

Average peak area Aw

 

f

Average f

 

1

13.02

25-0.1-10-1-10

0.00052

59232

58900

59066

0.00000877

0.00000912

25-0.2-10-1-10

0.00104

109771

109330

109550.5

0.00000946

 

2

13.02

25-0.1-10-1-10

0.00052

59660

59920

59790

0.00000893

0.00000926

25-0.2-10-1-10

0.00104

111332

111262

111297

0.00000959

 

3

14.05

25-0.1-10-1-10

0.00056

60854

60711

60782.5

0.00000920

0.00000952

25-0.2-10-1-10

0.00112

113999

113124

113561.5

0.00000985

 

Table 3: Study solution in water

 

Sample day/ flask

Weight of the

sample [mg]

 

Dilution [mL]

 

Cp [mg/mL]

 

Peak area

Average peak area

Ap

 

X

[g/kg]

1

1

507.23

10

50.7230

79480

79361

79420.5

0.0143

2

505.93

50.5930

77976

78115

78045.5

0.0141

2

3

495.42

10

49.5420

76487

76583

76535

0.0143

4

470.68

47.0680

73739

73079

73409

0.0144

3

5

498.98

10

49.8980

73301

72178

72739.5

0.0139

6

497.28

49.7280

73797

73557

73677

0.0141

Conclusions:
The water solubility of the test item was found to be 0.0142 g/l at 20ºC.
Executive summary:

The water solubility of the test item was determined using the shake flask method according to OECD 105 and EU method A.6, under GLP conditions. After a preliminary test to determine the necessary concentration to saturate 70 mL of water, three 100 ml beakers were filled with 5 times this concentration. The three flasks were centrifuged for one day at 20ºC, and two and three days at 30ºC respectively. The concentration of the test substance was measured with a validated HPLC method and since results of the three vessels did not differ by more than 15% the test was considered satisfactory. Two replicate measurements were performed. The water solubility of the test item was 0.0142 g/l at 20 ± 0.5°C (pH 4.99 -5.09).

Description of key information

Key study. Method according to OECD 105/ EU A.6, GLP study. The water solubility of the test item was 0.0142 g/l at 20ºC.

Key value for chemical safety assessment

Water solubility:
0.014 g/L
at the temperature of:
20 °C

Additional information