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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation
Justification for type of information:
Experimental study made available by ECHA
Qualifier:
according to
Guideline:
other: 92/69/EEC, C3 OECD 201
Principles of method if other than guideline:
Toxicity to aquatic algae and cyanobacteria study of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) for 72 hrs.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material (IUPAC name): (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS)
- Common name: Lokanol
- Molecular formula: C15H26O
- Molecular weight: 222.3694 g/mol
- InChl: 1S/C15H26O/c1-13(2)10(6-12-8-15(12,13)4)5-11-7-14(11,3)9-16/h10-12,16H,5-9H2,1-4H3
- Substance type: Organic
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Acetone (used as a pre-solvent)
0.01%
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Hardness:
24 mg CaCO3/L
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
0.53 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.24 mg/L
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.24 mg/L
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
%Concentration loss over test: 90 ... 6
Validity criteria fulfilled:
not specified
Conclusions:
Toxicity of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) on Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum) algae was determined by obatining EC50 and NOEC value. Based on the reduction rate of Pseudokirchneriella subcapitata, due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) for 72 hrs, the EC50 value was 0.53 mg/l and the NOEC was 0.24 mg/l and inhibition of growth rate of Pseudokirchneriella subcapitata for 72 hours the EC50 value was 1 mg/l and the NOEC was 0.24 mg/l.
Executive summary:

Study was performed to determine short term toxicity and Growth Inhibition of Alga ( Pseudokirchneriella subcapitata) due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) according to the other guideline 92/69/EEC, C3 OECD 201 in the static system.

Exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) was given to Pseudokirchneriella subcapitata alga for 72 hrs and auxilary solvent used for the study was 0.01 % acetone and EC50 and NOEC value was obtained.

Based on the reduction rate of Pseudokirchneriella subcapitata, due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) for 72 hrs, the EC50 value was 0.53 mg/l and the NOEC was 0.24 mg/l and inhibition of growth rate of Pseudokirchneriella subcapitata for 72 hours the EC50 value was 1 mg/l and the NOEC was 0.24 mg/l.

Based on the EC50 of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS), it was concluded that the test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) was consider as toxic and can be consider to be classified as aquatic chronic /acute 1 category as per CLP classification criteria.

Description of key information

Study was performed to determine short term toxicity and Growth Inhibition of Alga ( Pseudokirchneriella subcapitata) due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) according to the other guideline 92/69/EEC, C3 OECD 201 in the static system.

Exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) was given to Pseudokirchneriella subcapitata alga for 72 hrs and auxilary solvent used for the study was 0.01 % acetone and EC50 and NOEC value was obtained.

Based on the reduction rate of Pseudokirchneriella subcapitata, due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) for 72 hrs, the EC50 value was 0.53 mg/l and the NOEC was 0.24 mg/l and inhibition of growth rate of Pseudokirchneriella subcapitata for 72 hours the EC50 value was 1 mg/l and the NOEC was 0.24 mg/l.

Based on the EC50 of test chemical(1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS), it was concluded that the test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS)was consider as toxic and can be consider to be classified as aquatic chronic /acute 1 category as per CLP classification criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:
0.53 mg/L
EC10 or NOEC for freshwater algae:
0.24 mg/L

Additional information

Data available for the target chemical was reviewed to determine short term toxicity and Growth Inhibition of Alga due to the exposure of test chemical

Butyl (dialkyloxy(dibutoxyphosphoryloxy))titanium(trialkyloxy)titanium phosphate. The studies are as mentioned below:

Study was performed to determine short term toxicity and Growth Inhibition of Alga ( Pseudokirchneriella subcapitata) due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) according to the other guideline 92/69/EEC, C3 OECD 201 in the static system.

Exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) was given to Pseudokirchneriella subcapitata alga for 72 hrs and auxilary solvent used for the study was 0.01 % acetone and EC50 and NOEC value was obtained.

Based on the reduction rate of Pseudokirchneriella subcapitata, due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) for 72 hrs, the EC50 value was 0.53 mg/l and the NOEC was 0.24 mg/l and inhibition of growth rate of Pseudokirchneriella subcapitata for 72 hours the EC50 value was 1 mg/l and the NOEC was 0.24 mg/l.

The above study was supported by another study which was performed to determine short term toxicity and Growth Inhibition of Alga ( Pseudokirchneriella subcapitata) due to the exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) according to the other guideline 92/69/CE, C3 in the static system.Exposure of test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS) was given to Pseudokirchneriella subcapitata alga for 72 hrs and EC50 and NOEC value was obtained.Based on the reduction and inhibition of growth rate of green algae due to the exposure with test chemical, the EC50 was 0.33 and 0.14 mg/l and the NOEC was 0.74 and 0.14 mg/l.

Based on the EC50 of test chemical(1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS), it was concluded that the test chemical (1-METHYL-2-(1,2,2-TRIMETHYLBICYCLO(3.1.0)-HEX-3-YLMETHYL)CYCLOPROPYL)METHANOL( MIXTURE OF DIASTEREOISOMERS)was consider as toxic and can be consider to be classified as aquatic chronic /acute 1 category as per CLP classification criteria.