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EC number: 944-860-3 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Study period:
- 07/03/2018-08/03/2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification
- Qualifier:
- according to guideline
- Guideline:
- other: REACH Guidance on QSARs R.6 (following OCDE 201, EU C.3)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent glucose Smile : C([C@@H]1[C@H]([C@@H]([C@H](C(O1)O)O)O)O)O
- Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- not specified
- Details on test solutions:
- Not applicable
- Test organisms (species):
- other: Pseudokirchneriella subcapitata, Desmodesmus subspicatus, Scenedesmus quadricauda
- Details on test organisms:
- Not applicable
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 d
- Remarks on exposure duration:
- Not applicable
- Post exposure observation period:
- Not applicable
- Hardness:
- Not applicable
- Test temperature:
- Not applicable
- pH:
- Not applicable
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Not applicable
- Details on test conditions:
- Not applicable
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 358 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: QSAR predicted value
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- 95% confidence limits (g test item.L-1) : 314 - 408
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to algae of glucose is : 358 g/L
Based on a constituent approach, the toxicity to algae of EXTRACT OF ALLIUM CEPA (AMARYLLIDACEAE) BULB AND CITRUS LIMON (RUTACEAE) PULP AND SODIUM CHLORIDE is considered as greater than 88 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Study period:
- 07/03/2018-08/03/2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification
- Qualifier:
- according to guideline
- Guideline:
- other: REACH Guidance on QSARs R.6 (following OCDE 201, EU C.3)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent citric acid Smile : C(C(=O)O)C(CC(=O)O)(C(=O)O)O
- Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- not specified
- Details on test solutions:
- Not applicable
- Test organisms (species):
- other: Pseudokirchneriella subcapitata, Desmodesmus subspicatus, Scenedesmus quadricauda
- Details on test organisms:
- Not applicable
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 d
- Remarks on exposure duration:
- Not applicable
- Post exposure observation period:
- Not applicable
- Hardness:
- Not applicable
- Test temperature:
- Not applicable
- pH:
- Not applicable
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Not applicable
- Details on test conditions:
- Not applicable
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 0.088 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: QSAR predicted value
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- 95% confidence limits (g test item.L-1) : 0.052 – 0.149
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to algae of citric acid is : 0.088 g/L
Based on a constituent approach, the toxicity to algae of EXTRACT OF ALLIUM CEPA (AMARYLLIDACEAE) BULB AND CITRUS LIMON (RUTACEAE) PULP AND SODIUM CHLORIDE is considered as greater than 88 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- (Q)SAR
- Adequacy of study:
- key study
- Study period:
- 07/03/2018-08/03/2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- results derived from a valid (Q)SAR model, but not (completely) falling into its applicability domain, with adequate and reliable documentation / justification
- Qualifier:
- according to guideline
- Guideline:
- other: REACH Guidance on QSARs R.6 (following OCDE 201, EU C.3)
- GLP compliance:
- no
- Specific details on test material used for the study:
- Constituent sucrose Smile : C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@]2([C@H]([C@@H]([C@H](O2)CO)O)O)CO)O)O)O)O
- Analytical monitoring:
- not required
- Details on sampling:
- Not applicable
- Vehicle:
- not specified
- Details on test solutions:
- Not applicable
- Test organisms (species):
- other: Pseudokirchneriella subcapitata, Desmodesmus subspicatus, Scenedesmus quadricauda
- Details on test organisms:
- Not applicable
- Test type:
- not specified
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 72 d
- Remarks on exposure duration:
- Not applicable
- Post exposure observation period:
- Not applicable
- Hardness:
- Not applicable
- Test temperature:
- Not applicable
- pH:
- Not applicable
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Not applicable
- Details on test conditions:
- Not applicable
- Reference substance (positive control):
- not required
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 269 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: QSAR predicted value
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- 95% confidence limits (g test item.L-1) : 236 – 307
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to algae of sucrose is : 269 g/L
Based on a constituent approach, the toxicity to algae of EXTRACT OF ALLIUM CEPA (AMARYLLIDACEAE) BULB AND CITRUS LIMON (RUTACEAE) PULP AND SODIUM CHLORIDE is considered as greater than 88 mg/L.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM 1988
- Principles of method if other than guideline:
- Results from : Buckley JA et al; Bull Environ Contam Toxicol 57 (6): 1003-1008 (1996) Available from, as of November 21, 2006:http://cfpub.epa.gov/ecotox/quick_query.htm
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Constituent glucose sodium chloride : [Na+].[Cl-]
- Analytical monitoring:
- not required
- Vehicle:
- yes
- Details on test solutions:
- Hoagland’s medium (ASTM 1988) modified by the omission of sucrose, yeast extract and bactotryptone, adjusted to pH 5.0 and incubated at 24 ± l°C with a 24 hr light period of 3100 to 3700 Lux.
- Test organisms (species):
- other: Lemna minor
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
- pH:
- 7
- Nominal and measured concentrations:
- The concentrations used were 0 (control), 2.59, 4.32, 7.2 and 12.0 g/L
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 4.88 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- 95% confidence limits (g test item.L-1) : 3.95 – 6.02
- Validity criteria fulfilled:
- yes
- Executive summary:
The toxicity to algae of sodium chloride is : 4.88g/L
Based on a constituent approach, the toxicity to algae of EXTRACT OF ALLIUM CEPA (AMARYLLIDACEAE) BULB AND CITRUS LIMON (RUTACEAE) PULP AND SODIUM CHLORIDE is considered as greater than 88 mg/L.
Referenceopen allclose all
Description of key information
The growth inhibition of algae was determined using validated QSAR models for the Mechanisms of Action (MechoA) associated to the constituents, i.e. MechoA 1.1: baseline toxicity (non-polar narcosis) and MechoA 5.2: carboxylic acids. The QSAR model is based on validated data derived from 72-hour ErC50 test on algae, for which the concentrations of the test item had been determined by chemical analyses over the test period.
Based on a constituent approach, the toxicity to algae of EXTRACT OF ALLIUM CEPA (AMARYLLIDACEAE) BULB AND CITRUS LIMON (RUTACEAE) PULP AND SODIUM CHLORIDE is considered as greater than 88 mg/L (which is the lowest EC50 among the constituants of this extract).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 88 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
