(1Z)-1-Chloro-2,3,3-trifluoroprop-1-ene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29/11/2016 - 17/02/2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

EC Number:

824-458-3

Cas Number:

1263679-68-0

Molecular formula:

C3H2ClF3

IUPAC Name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The test solutions were prepared according to the methods described in Appendix 3. Test solutions, approximately 100 mL, were poured into each of the five vessels per test group and immediately plugged with a ground-in stopper to prevent volatilization of the test substance. The volumes of the test solution in the vessels were adjusted to 100 mL by plugging. One of five vessels of each test group was used for measuring the test conditions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Scientific name: Daphnia magna

Source: National Institute for Environmental Studies, Japan

Date of receipt: July 18, 1995 (The daphnids have been maintained at the testing facility).

Sensitivity to the reference substance: Acute immobilisation tests of the reference substance (potassium dichromate, guaranteed reagent) have been conducted periodically (approximately every 6 months).

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

228 mg CaC03/L

Test temperature:

20±1 °C

pH:

8.3

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

39.7 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10.8 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

123 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

mobility

Details on results:

In the control, the percentage of immobilisation was 0% at the end of exposure, and no daphnids were observed floating on the surface of the water.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

An acute immobilisation test of the test substance with Daphnia magna was conducted in order to determine the median effect concentration (EC50) of the test substance for 48-hour exposure period. The 48-hour median effect concentration (EC50) was 39.7 mg/L (95% confidence limits: 33.5-47.6 mg/L).

Executive summary:

An acute immobilisation test of the test substance with Daphnia magna was conducted in order to determine the median effect concentration (EC50) of the test substance for 48-hour exposure period. The 48-hour median effect concentration (EC50) was 39.7 mg/L (95% confidence limits: 33.5-47.6 mg/L).