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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29/11/2016 - 17/02/2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2017
Report Date:
2017

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
The test solutions were prepared according to the methods described in Appendix 3. Test solutions, approximately 100 mL, were poured into each of the five vessels per test group and immediately plugged with a ground-in stopper to prevent volatilization of the test substance. The volumes of the test solution in the vessels were adjusted to 100 mL by plugging. One of five vessels of each test group was used for measuring the test conditions.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Scientific name: Daphnia magna

Source: National Institute for Environmental Studies, Japan

Date of receipt: July 18, 1995 (The daphnids have been maintained at the testing facility).

Sensitivity to the reference substance: Acute immobilisation tests of the reference substance (potassium dichromate, guaranteed reagent) have been conducted periodically (approximately every 6 months).

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
48 h

Test conditions

Hardness:
228 mg CaC03/L
Test temperature:
20±1 °C
pH:
8.3

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
39.7 mg/L
Nominal / measured:
meas. (initial)
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
10.8 mg/L
Nominal / measured:
meas. (initial)
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
123 mg/L
Nominal / measured:
meas. (initial)
Basis for effect:
mobility
Details on results:
In the control, the percentage of immobilisation was 0% at the end of exposure, and no daphnids were observed floating on the surface of the water.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
An acute immobilisation test of the test substance with Daphnia magna was conducted in order to determine the median effect concentration (EC50) of the test substance for 48-hour exposure period. The 48-hour median effect concentration (EC50) was 39.7 mg/L (95% confidence limits: 33.5-47.6 mg/L).
Executive summary:

An acute immobilisation test of the test substance with Daphnia magna was conducted in order to determine the median effect concentration (EC50) of the test substance for 48-hour exposure period. The 48-hour median effect concentration (EC50) was 39.7 mg/L (95% confidence limits: 33.5-47.6 mg/L).