(1Z)-1-Chloro-2,3,3-trifluoroprop-1-ene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26/11/2016 - 17/02/2017

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

EC Number:

824-458-3

Cas Number:

1263679-68-0

Molecular formula:

C3H2ClF3

IUPAC Name:

(1Z)-1-chloro-2,3,3-trifluoroprop-1-ene

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Samples of the test solutions at the beginning and the end of the renewal period, i.e. when it is freshly prepared and immediately prior to renewal or at the end of exposure are referred to as one set. The two sets of test solutions, one at the beginning of exposure (0 hour) and before renewal at 24 hours and the other after renewal at 72 hours and at the end of exposure (96 hours), were analysed. The test solution of each test group was collected and analysed by gas chromatography/mass spectrometry (GC/MS).

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

Common name: Medaka

Scientific name: Oryzias latipes

Source: Breeding in the testing facility

Date of starting the collection of eggs: June 20, 2016

Lot No.: 16-M-0620B

Size: Total length: Recommended: 2.3 ± 1.2 cm; Measurements: 2.66 cm (2.39 - 3.03 cm), n=lO
Body weight: Measurements: 0.159 g (0.089- 0.241 g), n=lO; (Measurements of fish in the control group were made post-exposure)

Sensitivity to reference substance: The sensitivity of test fish has been confirmed in each lot by the acute toxicity test of reference substance (CuS04(II)· 5H20, guaranteed reagent). The 96-hour median lethal concentration (96-hour LC50, in anhydrous) is shown below:
- 96-hour LC50 of the lot concerned (Date of performing the acute toxicity test: October 7, 2016)
- 0.26 mg/L (95% confidence limits: 0.10 - 0.40 mg/L)
-The average LC50 value in this laboratory (n = 80, since 1995); Average± standard deviation: 0. 73 ± 0.29 mg/L; Minimum value - maximum value: 0.28 - 1.5 mg/L

Age: Less than a half year after hatching

Medication before acclimation: Applied (New Green F, San-ei Pharmaceutical Co. Ltd.)

Acclimation period: November 24, 2016 - December 5, 2016

Acclimation conditions:
- Water: Dechlorinated water
- Procedure: Flow-through (Biomass loading: 1 g fish/L/day or less)
- Temperature: 24 ± 1 °C
- Dissolved oxygen concentration (D.O.): 80% or more of the saturation
- pH: 6.0-8.5
- Light: Fluorescent light, 16-hour light (1000 lux or less) I 8-hour dark
- Feed: TetraMin, TetraWerke
- Feeding amount: 2% of the body weight/day (approx.) (until 24 hours before exposure)
- Medication during acclimation: Not applied
- Mortality during 7 days before the test: Less than 5%

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

47 mg CaCO3/L

Test temperature:

23.7 - 24.0

pH:

6.8 - 7.5

Dissolved oxygen:

5.0 - 8.8 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

110 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

76.1 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Acute toxicity test of the test substance with Medaka ( Oryzias latipes) was conducted using a binomial method in order to determine the median lethal concentration (LC50) of the test substance for 96-hour exposure. This was determined to be 110 mg/L (95% confidence limits: 76.1 - ∞ mg/L by binomial method).

Executive summary:

Acute toxicity test of the test substance with Medaka ( Oryzias latipes) was conducted using a bonomial method in order to determine the median lethal concentration (LC50) of the test substance for 96-hour exposure. This was determined to be 110 mg/L (95% confidence limits: 76.1 - ∞ mg/L)