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EC number: 801-347-8
CAS number: 20445-94-7
Tributyl(ethyl) phosphonium diethylphosphate
appeared to be non corrosive to the skin, while it induced serious eye
damange in the Bovine Corneal Opacity and Permeability test.
An in vitro skin corrosion test of
Tributyl(ethyl) phosphonium diethylphosphate was performed in a human
three dimensional epidermal model (EpiDerm) according to the OECD No.
431 guideline and GLP. The possible corrosive potential of the test item
was tested through topical application for 3 minutes and 1 hour. The
test item was applied undiluted (50 μL) directly on top of the skin
Exposure of the test item was terminated by
rinsing with Phosphate Buffered Saline (PBS). The viability of each disk
was assessed by incubating the tissues for 3 hours with MTT solution in
an incubator with 5% CO2 protected from light. The precipitated formazan
crystals were then extracted using acidified isopropanol and quantified
The positive control had a mean relative
tissue viability of 6.3% after the 1-hour exposure. The absolute mean
OD570 (optical density at 570 nm) of the negative control tissues was
within the acceptance limits of OECD 431 and the laboratory historical
control data range. In the range of 20 - 100% viability the Coefficient
of Variation between tissue replicates was < 13%, indicating
that the test system functioned properly.
Skin corrosion is expressed as the remaining
cell viability after exposure to the test item. The relative mean tissue
viability obtained after 3-minute and 1-hour treatments with the test
item compared to the negative control tissues was 88% and 18%,
respectively. Because the mean relative tissue viability for the test
item was not below 50% after the 3-minute treatment and not below 15%
after the 1-hour treatment the test item is considered to be not
A GLP compliant in-vitro study was performed
according to OECD Guideline 437 in order to evaluate the ocular
irritation properties of Tributyl(ethyl) phosphonium diethylphosphate on
an isolated bovine cornea. The possible ocular irritancy of the test
item was tested through topical application for approximately 10
minutes. For each treatment group (negative control, positive control
and test item), three replicates were used. After removal of the test
item and two hours post-incubation, opacity and permeability values were
measured. After treatment, the IVIS (in vitro irritancy score) in the
controls is within the acceptability criterion. The positive control
showed clear irritating effects. Tributyl(ethyl) phosphonium
diethylphosphate induced serious eye damage through both endpoints,
resulting in a mean in vitro irritancy score of 59 after 10 minutes of
Based on the results of the in vitro EpiDerm
Skin Model test, Tributyl(ethyl) phosphonium diethylphosphate
is non-corrosive to skin. Taking into account the experimental results, a
classification as irritating to skin is proposed based on precautionary principle.
A GLP compliant in-vitro study according to
OECD Guideline 437 was performed in order to evaluate the ocular
irritation properties of Tributyl(ethyl) phosphonium diethylphosphate
on isolated bovine cornea. Since Tributyl(ethyl) phosphonium
diethylphosphate induced an IVIS > 55, it is concluded that the
substance induced serious eye damage in the Bovine Corneal Opacity and
Based on the experimental data,
Tributyl(ethyl) phosphonium diethylphosphate is classified as Cat
2; H315 "irritating to skin" and Cat.
1; H318 'Causes serious eye damage'
in accordance with the EU Classification,
Labelling and Packaging of Substances and Mixtures (CLP) Regulation (EC)
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