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Diss Factsheets

Administrative data

Endpoint:
developmental toxicity
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
BG Chemie - Potential Health Hazards of Existing Chemiclas (TOXIKOLOGISCHE BEWERTUNG zu Benzolsulfonsäurehydrazid, BG-Nr. 220)
Author:
Berufsgenossenschaft Rohstoffe und chemische Industrie (BG RCI)
Reference Type:
publication
Title:
Toxicity of rubber chemicals towards three-day chicken embryos
Author:
Korhonen A, Hemminki K, Vainio H
Year:
1983
Bibliographic source:
Scand J Work Environ Health 1983;9(2):115-119

Materials and methods

GLP compliance:
not specified

Test animals

Species:
other: chicken
Strain:
other: white Leghorn
Details on test animals or test system and environmental conditions:
3 day old chicken embryos (white Leghorn)

Administration / exposure

Route of administration:
infusion
Vehicle:
acetone
Details on exposure:
75% BSH in paraffin oil dissolved in acetone was administered at concentrations of 1.0, 2.0, 3.0 and 4.0 µmol/egg into the air chamber of 3 day old chicken embryos (white Leghorn). The injection volume was 1 µl.
Duration of treatment / exposure:
11 days
Duration of test:
14-day incubation period
No. of animals per sex per dose:
30 eggs were used for each concentration.
Control animals:
yes, concurrent vehicle
Details on study design:
At the end of the 14-day incubation period (11 days after administration), the embryotoxic potency was evaluated according to the total number of embryos affected 11 days after administration, using overall mortality and the number of malformed embryos (total effect) as a basis.

Results and discussion

Results (fetuses)

Effect levels (fetuses)

Key result
Dose descriptor:
other: median effect dose (ED50)
Effect level:
2.6 other: µmol/egg
Based on:
test mat.
Basis for effect level:
other: malformations and mortality

Any other information on results incl. tables

At 1.0 µmol, 13% of the treated eggs were affected, at 2.0 and 3.0 µmol 47% and at 4.0 µmol this figure was 70%. From these vlaues, an ED50 (total effect) of 2.6 µmol/egg (confidence range 2.1 to 3.3 µmol) was determined over the 11 days following application. The malformations were described as 2 different types of eye defect and (reduced eye cup and partial or complete absence of lids and cornea), defects of the coelum wall and generalized oedema. The highest mortality rate (46%) and malformation rate (43%) determined occured in the (dead and surviving) embryos treated with 4.0 µmol/egg.

Applicant's summary and conclusion

Conclusions:
It is almost impossible to apply these findings to mammalian systems since the absorption barriers and detoxification mechanisms in the latter are not taken into account.