Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12 August 2019 - 15 August 2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: hatched from ephippia sourced from Eco Cheshire Solutions
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Observations of test animals were carried out at 48h and at 24h for the definitive and reference tests
Test temperature:
20 °C ± 2 °C Constant within 1 °C
pH:
6 - 9
Dissolved oxygen:
>3 mg/L
Nominal and measured concentrations:
Nominal concentrations: 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 100 mL capacity borosilicate glass crystallising dishes
- Type: closed
- Volume of solution: 50 mL
- Aeration: No aeration during the 48 hour study
- Type of flow-through: static
- No. of organisms per vessel: 10 animals per vessel
- No. of vessels per concentration (replicates): 2 vessels per concentration
- No. of vessels per control (replicates): 2 vessels per control
- No. of vessels per vehicle control (replicates): 2 vessels per control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO reconstituted water (as specified in OECD 202)
- Intervals of water quality measurement: Carried out at each concentration at 0h and 48h

EFFECT PARAMETERS MEASURED: Immobilisation
OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark

RANGE-FINDING STUDY
- Test concentrations: 1000, 100, 10 and 1 mg/L
- Results used to determine the conditions for the definitive study: yes
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
> 10 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: Yes
- Limit test: No
- Dose-response test: Yes
- 24h EC50: 1.4 mg/L
Reported statistics and error estimates:
The result was based on nominal concentrations and was calculated by Linear Interpolation within the CETIS suite of statistical analysis. There were <10% control mortality observed throughout the definitive test.

Nominal

concentration

(mg/l)

Number dead

immobile at 24h

Number dead/immobile

at test termination (48h)

Total

exposed

Replicate a

Replicate b

Replicate a

Replicate b

1000

0

0

0

0

20

1800

0

0

0

0

20

3200

0

0

0

0

20

5600

0

0

2

0

20

10000

0

0

2

0

20

Validity criteria fulfilled:
yes
Conclusions:
The 48-hour EC50 was 10,000 mg/L and the 48-hour NOEC 10,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.
Executive summary:

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to aquatic invertebrates was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

The species was Daphnia magna.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1000, 1,800, 3,200, 5,600 and 10,000mg/L were used during the definitive test.

The 48-hour EC50 was 10,000 mg/L and the 48-hour NOEC 10,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Description of key information

The short-term toxicity of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid (EC 948-054-2) to aquatic invertebrates was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

The species was Daphnia magna.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 1000, 1,800, 3,200, 5,600 and 10,000mg/L were used during the definitive test.

The 48-hour EC50 was 10,000 mg/L and the 48-hour NOEC 10,000 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10 g/L

Additional information