Acrylate of bisphenol A-type epoxy resin

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): local wastewater treatment plant, ARA Birs (Birsfelden / Switzerland)
- Preparation of inoculum for exposure: The aerobic activated sewage sludge was washed three times by centrifugation, decantation of the supernatant (liquid phase) and resuspension of the solid material in tap water and finally in mineral medium. Aliquots of the homogenised final sludge suspension were weighed and dried (using a halogen moisture analyzer HS153 from Mettler Toledo).
- Concentration of sludge: Final concentration of 30 mg dry material per liter

Duration of test (contact time):

28 d

Initial conc.:

ca. 100 mg/L

Based on:

COD

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Mineral medium was prepared according to the testing guidelines
- Test temperature: 22.8 to 23.3 °C
- pH: Prior to the start of the test (Day 0), the pH was measured in each test vessel before the addition of the inoculum, and was found to be 7.4 for all vessels.
- pH adjusted: no
- Continuous darkness: no

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Measuring equipment: For the measurement of the BOD, the OxiTop® Control system from WTW GmbH, Weilheim, Germany consisting of pressure measuring heads, test bottles, inductive stirring system and the OxiTop® OC 110 Controller was used.

SAMPLING
Not applicable: BOD values were automatically recorded every four hours

CONTROL AND BLANK SYSTEM
- Inoculum blank: Included
- Abiotic sterile control: Not included
- Toxicity control: Included

Reference substance:

benzoic acid, sodium salt

Preliminary study:

Due to the complex structure of the test item, its Chemical Oxygen Demand (COD) was determined according to the EU Commission Directive 92/69/EEC, Part C.6 following DIN 38414-S9.

Key result

Parameter:

% degradation (O2 consumption)

Value:

5

Sampling time:

28 d

Parameter:

% degradation (O2 consumption)

Value:

4.5

Sampling time:

21 d

Parameter:

% degradation (O2 consumption)

Value:

4.5

Sampling time:

14 d

Parameter:

% degradation (O2 consumption)

Value:

2

Sampling time:

7 d

Parameter:

COD

Value:

2.05 g O2/g test mat.

Results with reference substance:

The percent of biodegradation of sodium benzoate was calculated based on the ThOD of 1.67 mg O2/mg. In the procedure controls, the average biodegradation of the reference item sodium benzoate reached 61 % and 77 % by Day 4 and Day 14, respectively. At the end of the test (Day 28), the average biodegradation was 79 %.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid is not ready biodegradable.

Executive summary:

The ready biodegradability of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid was evaluated during a GLP-compliant study perform in accordance with the OECD Testing Guideline 301F (Manometric Respirometry).

The test was performed in closed vessels containing a known concentration of test item in inoculated medium incubated at constant temperature for 28 days. The percent of biodegradation of the test item was calculated based on the Chemical Oxygen Demand (COD) of 2.05 mg O2/mg test item.

Sodium benzoate was used as reference substance.

The Biochemical Oxygen Demand (BOD) of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid in the test media was minimal above the oxygen consumption in the inoculum controls during the whole incubation period. Therefore, the calculated mean biodegradation value of the test item at the end of the test period, on Day 28, was at only 5 % of the COD.

Oxygen consumption was evaluated through the study.

Validation criteria were met. A toxicity control indicated that the test substance had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 100 mg/L.

4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid was found to be not readily biodegradable under the test conditions within 28 days.

Description of key information

The ready biodegradability of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid was evaluated during a GLP-compliant study perform in accordance with the OECD Testing Guideline 301F (Manometric Respirometry).

The test was performed in closed vessels containing a known concentration of test item in inoculated medium incubated at constant temperature for 28 days. The percent of biodegradation of the test item was calculated based on the Chemical Oxygen Demand (COD) of 2.05 mg O2/mg test item.

Sodium benzoate was used as reference substance.

The Biochemical Oxygen Demand (BOD) of 4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid in the test media was minimal above the oxygen consumption in the inoculum controls during the whole incubation period. Therefore, the calculated mean biodegradation value of the test item at the end of the test period, on Day 28, was at only 5 % of the COD.

Oxygen consumption was evaluated through the study.

Validation criteria were met. A toxicity control indicated that the test substance had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 100 mg/L.

4,4’-isopropylidenediphenol, reaction products with 1-chloro-2,3-epoxypropane, mono, di and triesters with acrylic acid was found to be not readily biodegradable under the test conditions within 28 days.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater