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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Remarks:
HPLC/DAD
Vehicle:
yes
Remarks:
test item
Details on test solutions:
The test item was not soluble. A special solubilization protocol was carried out before the experimentation according to the OECD23 guidance document on aquatic toxicity testing of difficult substances and mixtures. This method consists in stirring each test item nominal concentrations in test medium during 24h, in the dark and 20±2°C.
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Test type:
semi-static
Water media type:
other: test medium
Limit test:
no
Total exposure duration:
72 h
Test temperature:
22 - 23°C
Nominal and measured concentrations:
10 - 32 - 102 - 328 and 1050 mg/L
Reference substance (positive control):
yes
Remarks:
K2Cr2O7
Key result
Duration:
72 h
Dose descriptor:
EL50
Remarks:
growth rate
Effect conc.:
> 1 050 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EL10
Remarks:
growth rate
Effect conc.:
732 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EL50
Remarks:
% yield inhibition
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: % yield inhibition
Key result
Duration:
72 h
Dose descriptor:
EL10
Remarks:
% yield inhibition
Effect conc.:
255 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks on result:
other: % yield inhibition
Details on results:
The validity criteria were successfully fulfilled.
Results with reference substance (positive control):
not specified
Validity criteria fulfilled:
yes
Conclusions:
The toxicity potential on aquatic algae was determined according to the OECD201 guideline. The results obtained for the test item on the toxicological endpoint "inhibition of growth rate" and "yield inhibition" were :
- EL50 (growth rate) - 72h > 1050 mg/L
- EL10 (growth rate) - 72h = 732 mg/L
- EL50 (yield) - 72h = 1000 mg/L
- EL10 (yield) - 72h = 255 mg/L
Regarding these results, the test item is not considered as toxic for aquatic algae.
Executive summary:

The toxicity potential on aquatic algae was determined according to the OECD201 guideline. The results obtained for the test item on the toxicological endpoint "inhibition of growth rate" and "yield inhibition" were :

- EL50 (growth rate) - 72h > 1050 mg/L

- EL10 (growth rate) - 72h = 732 mg/L

- EL50 (yield) - 72h = 1000 mg/L

- EL10 (yield) - 72h = 255 mg/L

Regarding these results, the test item is not considered as toxic for aquatic algae.

Description of key information

The toxicity potential on aquatic algae was determined according to the OECD201 guideline. The results obtained for the test item on the toxicological endpoint "inhibition of growth rate" and "yield inhibition" were:

- EL50 (growth rate) - 72h > 1050 mg/L

- EL10 (growth rate) - 72h = 732 mg/L

- EL50 (yield) - 72h = 1000 mg/L

- EL10 (yield) - 72h = 255 mg/L

Regarding these results, the test item is not considered as toxic for aquatic algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
1 050 mg/L

Additional information