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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
Name CARMINE LAKE
Batch no. HPFC16006A
Appearance fine red powder
Composition Hydrated aluminium chelate of carminic acid (7-beta-Dglucopyranosyl-
3,5,6,8-tetrahydroxy-1-methyl-9,10-dioxoanthracene-
2-carboxylic acid)
CAS No. 1390-65-4
EC-No. 217-724-4
Molecular formula C22H20O13
Molecular weight 492.39 g/mol
Purity 55 % Carmine
Homogeneity homogeneous
Vapour pressure unknown
Stability in solvents H2O: not stated; EtOH: not stated; Acetone: not stated;
CH3CN: not stated; DMSO: not stated
Solubility in solvents Soluble in alkaline water (pH >9.5); H2O: unknown; EtOH:
unknown; Acetone: unknown; CH3CN: unknown; DMSO: unknown
Storage Room Temperature (20 ± 5 °C); Keep away from light
Analytical monitoring:
yes
Vehicle:
yes
Details on test solutions:
CaCl2*2H2O: 293.80 mg/L (Ca 2+ 80.09 mg/L)
MgSO4*7H2O: 123.30 mg/L (Mg 2+ 12.16 mg/L)
NaHCO3: 64.80 mg/L (Na+ 17.73 mg/L)
KCl: 5.80 mg/L (K + 3.04 mg/L)
Test organisms (species):
Daphnia magna
Details on test organisms:
Species/Strain: Daphnia magna STRAUS / BERLIN
Sex: female
Age: between 0 and 24 hours
Vessels: preserving glasses, nominal volume 2 L
Medium: M4 medium (recipe of ELENDT)
Food: green algae (Desmodesmus subspicatus)
Medium renewal: twice a week
Photo period: 16/8 hours, using neon tubes
Temperature: 20  2 °C
Test type:
static
Water media type:
brackish water
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 ± 1 and 48 ± 1 hours
Test temperature:
20  2 °C
pH:
7.8 ± 0.2
Dissolved oxygen:
at least 3 mg/L
Nominal and measured concentrations:
100 mg/L (nominal)
Reference substance (positive control):
yes
Remarks:
Potassium dichromate (CAS 7778-50-9)
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Validity criteria fulfilled:
yes

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.3 mg/L

Additional information