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EC number: 302-446-4 | CAS number: 94109-09-8
Ecotoxicological information
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Manometric respirometry test OECD 301F
- Principles of method if other than guideline:
- - Principle of test:
Manometric Respirometry test OECD 301F
- Short description of test conditions: The biodegradability was assessed by measuring the consumption of oxygen by a microbial population (activated sludge) exposed to the test item under controlled conditions.
- Parameters analysed / observed: Comsumpton of oxygen - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tri(tridecyl) benzene-1,2,4-tricarboxylate
- EC Number:
- 302-446-4
- EC Name:
- Tri(tridecyl) benzene-1,2,4-tricarboxylate
- Cas Number:
- 94109-09-8
- Molecular formula:
- C48H84O6
- IUPAC Name:
- tri(tridecyl) benzene-1,2,4-tricarboxylate
- Test material form:
- liquid
- Details on test material:
- - Moisture content: <= 0.1%
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: lot P-2960F-10
- Expiration date of the lot/batch: November 2013
- Purity test date: > 99%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Final dilution of a dissolved solid, stock liquid or gel: the Concentration corresponded to a 20:1 ratio of silica gel to test item.
FORM AS APPLIED IN THE TEST (if different from that of starting material)
TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
OTHER SPECIFICS:
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture:
Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Name and location of sewage treatment plant where inoculum was collected: Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Pretreatment: Sludge was centrifuged, supernatant discarded, and sludge pellet re-suspended in mineral medium twice; sludge was brought up to the original volume; aerated in teh dark at test temperature until use
- Initial biomass concentration:0.02g/L
Study design
- Test type:
- other: aerobic
- Water media type:
- other: sludge
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 22.1 +- 0.8°C
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 2 L falt-bottomed glass bottles
- Type (delete if not applicable): open / closed closed
- Material, size, headspace, fill volume: test volume 1500 mL
- Aeration: no
- No. of vessels per concentration (replicates): 2 (inoculum blank and test item)
- No. of vessels per control (replicates): 1 (Reference Item and Toxicity control)
- No. of vessels per vehicle control (replicates): 1
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 26.5 mg/L
- Nutrients provided for bacteria: see report
OTHER TEST CONDITIONS
- Photoperiod: in the dark or dim light (except during short dailey readings)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : consumption of O2 - Reference substance (positive control):
- yes
- Remarks:
- Canola oil
Results and discussion
Effect concentrations
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 37 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- other: toxicity
- Details on results:
- - Blank controls oxygen uptake rate:
Canola Oil
- Coefficient of variation of oxygen uptake rate in control replicates:
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The percentage degradation of the toxicity control (containing both test and reference items) reached 73%. Given that the value seen in the toxicity control is similar to that of the reference item alone (67%), it is unlikely that the test item had a significant effect on the microorganisms ability to utilise the reference item for metabolism.
NOEC = 37 m/g/L
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