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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Manometric respirometry test OECD 301F
Principles of method if other than guideline:
- Principle of test: Manometric Respirometry test OECD 301F
- Short description of test conditions: The biodegradability was assessed by measuring the consumption of oxygen by a microbial population (activated sludge) exposed to the test item under controlled conditions.
- Parameters analysed / observed: Comsumpton of oxygen
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
EC Number:
302-446-4
EC Name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
Cas Number:
94109-09-8
Molecular formula:
C48H84O6
IUPAC Name:
tri(tridecyl) benzene-1,2,4-tricarboxylate
Test material form:
liquid
Details on test material:
- Moisture content: <= 0.1%
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: lot P-2960F-10
- Expiration date of the lot/batch: November 2013
- Purity test date: > 99%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Ambient temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: insoluble in water. The test and reference items were added suing silica gel as a solid matrix.
- Final dilution of a dissolved solid, stock liquid or gel: the Concentration corresponded to a 20:1 ratio of silica gel to test item.


FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Name and location of sewage treatment plant where inoculum was collected: Annacis Island domestic wastewater treatment pilot plant, Delta, BC
- Pretreatment: Sludge was centrifuged, supernatant discarded, and sludge pellet re-suspended in mineral medium twice; sludge was brought up to the original volume; aerated in teh dark at test temperature until use
- Initial biomass concentration:0.02g/L

Study design

Test type:
other: aerobic
Water media type:
other: sludge
Total exposure duration:
28 d

Test conditions

Test temperature:
22.1 +- 0.8°C
Details on test conditions:
TEST SYSTEM
- Test vessel: 2 L falt-bottomed glass bottles
- Type (delete if not applicable): open / closed closed
- Material, size, headspace, fill volume: test volume 1500 mL
- Aeration: no
- No. of vessels per concentration (replicates): 2 (inoculum blank and test item)
- No. of vessels per control (replicates): 1 (Reference Item and Toxicity control)
- No. of vessels per vehicle control (replicates): 1
- No. of vessels per abiotic control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 26.5 mg/L
- Nutrients provided for bacteria: see report

OTHER TEST CONDITIONS
- Photoperiod: in the dark or dim light (except during short dailey readings)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : consumption of O2
Reference substance (positive control):
yes
Remarks:
Canola oil

Results and discussion

Effect concentrations
Key result
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
ca. 37 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
other: toxicity
Details on results:
- Blank controls oxygen uptake rate: Canola Oil
- Coefficient of variation of oxygen uptake rate in control replicates:

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The percentage degradation of the toxicity control (containing both test and reference items) reached 73%. Given that the value seen in the toxicity control is similar to that of the reference item alone (67%), it is unlikely that the test item had a significant effect on the microorganisms ability to utilise the reference item for metabolism.
NOEC = 37 m/g/L

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