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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report date:
2011

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
EC Number:
302-446-4
EC Name:
Tri(tridecyl) benzene-1,2,4-tricarboxylate
Cas Number:
94109-09-8
Molecular formula:
C48H84O6
IUPAC Name:
tri(tridecyl) benzene-1,2,4-tricarboxylate
Test material form:
liquid
Details on test material:
- Moisture content: <= 0.1%
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Source : Lipo Chemicals Inc; Lot No: P-2960F-10
- Expiration date of the lot/batch: June 29, 2013
- Purity test date: > 99% (email dated February 17, 2011)

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: : Not data available
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: none

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
- Preliminary purification step (if any):
- Final dilution of a dissolved solid, stock liquid or gel:
- Final preparation of a solid:

FORM AS APPLIED IN THE TEST (if different from that of starting material)

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)

OTHER SPECIFICS:

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Source: in house culture; original culture was obtained from Aquatic BioSystems (Fort Collins, CO) on 17 August 2006 (identified by Vittor and Associates, Mobile, AL as D. magna; 14 July 2003)
- Feeding during test : none

ACCLIMATION
- Acclimation period:
- Acclimation conditions (same as test or not):
- Type and amount of food:
- Feeding frequency:
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration:
- Health/mortality:

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:

Study design

Test type:
static
Water media type:
other: reconstituted water
Total exposure duration:
48 h

Test conditions

Hardness:
Hardness of 92 mg/L as CaCO3
Test temperature:
19.9 to 21.9°C
pH:
7.8
Dissolved oxygen:
8.7 to 9.0 mg/L
Salinity:
Na/K ratio 25.2
Conductivity:
312 microS/cm
Nominal and measured concentrations:
100 mg test item/L (Limit test)
Details on test conditions:
TEST SYSTEM
- Test vessel:250 mL glass beakers covered with Plexiglass
- Material, size, headspace, fill volume: Test volume 200 mL
- Aeration: before use in the test
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Salinity:
- Culture medium different from test medium:
- Intervals of water quality measurement:

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

VEHICLE CONTROL PERFORMED: yes/no

RANGE-FINDING STUDY
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
yes

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
other: WAF
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The effect of the test item on < 24h old Daphnia magna neonates by observing the immobilisation of test organisms, over a 48h exposure period, under controlled conditions to 0 and 100 mg/L (WAF nominal concentrations) of the test item.
Results : 0% immobilisation observed in all of the replicates of the control and 100 mg test item/L nominal concentration.
EC50 (nominal) > 100 mg/L (48h)
LOEC (nominal) > 100 mg/L (48h)
NOEC (nomial) = 100 mg/L (48h)
Not harmful to Daphnia Magna

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