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Diss Factsheets
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EC number: 204-370-6 | CAS number: 120-11-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzyl 2-methoxy-4-prop-1-enylphenyl ether
- EC Number:
- 204-370-6
- EC Name:
- Benzyl 2-methoxy-4-prop-1-enylphenyl ether
- Cas Number:
- 120-11-6
- Molecular formula:
- C17H18O2
- IUPAC Name:
- benzyl 2-methoxy-4-prop-1-enylphenyl ether
Constituent 1
Test system
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- Approximately 50 mg of solid test item are evenly applied over the 0.6 cm2 of the tissue surface (approximately 83.3 mg/cm2 application).
- Duration of treatment / exposure:
- Tissues treated with solid test items and with control substances tested concurrently to solid test items are incubated for 6 ± 0.25 hours at standard culture conditions.
- Duration of post- treatment incubation (in vitro):
- At the end of the exposure period, the test item and control substances should be carefully removed from the tissue surface by extensive rinsing with Ca2+/Mg2+-free DPBS at room temperature. This rinsing step is followed by a 25 ± 2 minutes post-exposure immersion in fresh medium at room temperature and an 18 ± 0.25 hours post-exposure incubation in fresh medium at standard culture conditions, prior to performing the MTT assay.
- Number of animals or in vitro replicates:
- Two tissue replicates are used for each test item and each control substance.
- Details on study design:
- 37°C, 5% CO2, 95% humidity (standard culture conditions)
Results and discussion
In vitro
Results
- Irritation parameter:
- other: tissue viability percent
- Value:
- 154.12
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the experimental conditions adopted and in accordance with the Regulation EC No. 1272/2008, the test item BENZYL ISO EUGENOL does not require classification for eye irritation or serious eye damage. It corresponds to the UN GHS No Category.
- Executive summary:
The aim of the study was to evaluate the eye hazard potential of the test item BENZYL ISO EUGENOL after topical administration on in vitro reconstructed human cornea-like epithelium tissues (EpiOcularTM tissue model).
The test item BENZYL ISO EUGENOL was applied, as supplied, at the dose of 50 mg to 2 DPBS pre-treated RhCE (EpiOcularTM tissue model) during 6 hours at 37°C, 5% CO2, 95% humidity (standard culture conditions). The exposure period was followed by extensive rinsing with DPBS at room temperature, a 25 minutes post-exposure immersion period at room temperature and 18 hours and 00 minute post-exposure incubation at standard culture conditions. The tissue viability was measured by performing an MTT assay. The experimental protocol was established in accordance with O.E.C.D. Test Guideline No. 492 adopted 09 October 2017.
The mean percent tissue viability of the RhCE replicates treated with the test item BENZYL ISO EUGENOL was 154.12% (considered as 100%), versus 44.64% in the positive control (Methyl acetate).
In conclusion, under the experimental conditions adopted and in accordance with the Regulation EC No. 1272/2008, the test item BENZYL ISO EUGENOL does not require classification for eye irritation or serious eye damage. It corresponds to the UN GHS No Category.
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