Toluene-2-sulphonamide

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

1997

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Sampling method: The concentration of the test substance in the test solution was measured at the beginning and the end of the exposure. No further details.

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: National Institute for Environmental Studies (Japan)

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

55 - 80 mg/L(as CaCO3)

Test temperature:

19.6 - 20.1 ºC

pH:

7.9 - 8.2

Dissolved oxygen:

6.6 - 8.5 mg/L

Conductivity:

171 mS/cm

Nominal and measured concentrations:

- Nominal concentrations: 0 (control), 10, 22, 46, 100 mg/L (geometric ratio 2.2)
- Measured concentrations: 0, 10, 23, 49, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: glass beaker, 100 ml, volume 80 mL per vessel
- Renewal rate of test solution (frequency/flow rate): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Total organic carbon: COD 0.2 mg/L.
- Hardness 55.6 mg/L (as CaCO3)
- Alkalinity: 37.5 mg/L
- pH: 7.7
- Sodium: 14.9 mg/L
- Potassium: 3.76 mg/L
- Conductivity: 171 mS/cm

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: 16 hours light - 8 hours dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility, reproduction

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2

Reference substance (positive control):

not required

Key result

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

immobilisation

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

79 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

49 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

100 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

- Concentrations: Measured concentrations were adopted because percentage of those concentrations for nominal concentrations exceeded ± 20 %.
- No parent daphnids died during the test, neither in the control nor in the test concentrations.
- Time (days) of the First Brood Production: Mean;Control (7 days), 10 mg/L (7 days), 23 mg/L (7 days), 49 mg/L (7 days) and 100 mg/L (7 days).
- Mean Cumulative Number of Offsprings Produced per Adult: 21days; Control (112.9), 10 mg/L (140.6), 23 mg/L (140.8), 49 mg/L (122.5) and 100 mg/L (27.9)

Reported statistics and error estimates:

- Data Analysis: for LC50; Not described, Dunnett test for NOEC
- Method of Calculating Mean Measured Concentrations (i.e. arithmetic mean, geometric mean, etc.): Time-weighted mean
- NOEC was determined based on the cumulative number of juveniles produced per adult alive for 21 days.

Table 1. Nominal and measured concentrations

Nominal Concentration [mg/L]	Measured concentration [mg/L] (% nominal)						Time-weighted mean for 21 days [mg/L] (% nominal)
	2 day new	5 day old	9 day new	12 day old	16 day new	19 day old
Control	< 0.07	< 0.07	< 0.07	< 0.07	< 0.07	< 0.07	--
10	11(110)	9.8(98)	8.0(80)	12(120)	11(110)	9.5(95)	10(100)
22	26(120)	22(100)	19(86)	23(100)	26(120)	21(95)	23(100)
46	61(130)	48(100)	40(87)	52(110)	50(110)	43(93)	49(110)
100	120(120)	100(100)	83(83)	100(100)	110(120)	95(95)	100(100)

 

Table 2. Effect Data (reproduction):

Concentration [mg/L]	Cumulative number of deaths, parental daphnids [days]
	0	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
Control	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
10	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
22	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
46	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
100	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0

 

Validity criteria fulfilled:

yes

Conclusions:

The test substance is not chronically toxic to invertebrates. The NOEC for reproduction is 49 mg/L.

Executive summary:

A Daphnia magna Reproduction Test was performed with the test item according to OECD 202, under GLP conditions. 10 daphnids per concentration (10 vessels with 1 animal each) were exposed to 0 (control), 22, 46, or 100 mg/L (nominal concentrations) of test item for 21 days at 20 ± 1 ºC, under semi-static conditions (renewal rate 3 times per week). The test item concentration was measured by GC-MS and, since the measured concentrations were outside the range ± 20 % of the nominal, all results were given as measured values. The 21d-LC50 of the test item in daphnia was determined to be >100 mg/L, based on parent mortality; the 21d-EC50 was 79 mg/L, based on reproduction; the NOEC was 49 mg/L and the LOEC 100 mg/L (measured concentrations).

Description of key information

Key study. Method according to OECD 202, GLP study. The 21d-LC50 of the test item in daphnia was determined to be >100 mg/L, based on parent mortality; the 21d-EC50 was 79 mg/L, based on reproduction; the NOEC was 49 mg/L and the LOEC 100 mg/L (measured concentrations).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

49 mg/L