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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Start: 02/07/2018 - End: 09/07/2018
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes
Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Green alga
- Strain: SAG 61:81
- Source: laboratory culture


ACCLIMATION
- Acclimation period:
- Culturing media and conditions: Culture medium was kept in 250ml Erlenmeyer flasks in the following conditions:
-Temperature: 23° ± 2°C
- Lighting: lighting greater than 4300 lux
- Sirring: steady with electrical stirrer
Test type:
static
Limit test:
yes
Total exposure duration:
72 h
Test temperature:
23 ± 2°C
Refer to results table below
pH:
Refer to results table below
Nominal and measured concentrations:
100.0 mg/l (nominal concentration)
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Initial cells density: 10^4 cells/ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water
- Culture medium different from test medium: No


OTHER TEST CONDITIONS
- Light intensity and quality: Continuous - with flourescent lamp 8000 lux



TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 5 ten-fold dilutions from 100 mg/l to 0.01 mg/l.
- Results used to determine the conditions for the definitive study: Yes
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal

Stability of the test item:

The test item was considered stable because the measured concentrations of the test item at the end of the test has been maintained within ± 20% at the beginning of the test.

The test item resulted stable during the test, therefore the statistical analysis (EC50) has been performed using the nominal concnetration at T=0.

Nominal conc.

Measured conc (mg/l) T=0

Measured conc. (mg/l) T=72 H

% concentration variation

Stability

Treated

102.70

104.02

1.28

Stable

Algal density (cells/ml) in treated group

Time (h)

Treated group

1

2

3

4

5

6

0

1.0E+04

1.0E+04

1.0E+04

1.0E+04

1.0E+04

1.0E+04

24

7.7E+04

7.7E+04

8.0E+04

7.8E+04

7.5E+04

7.2E+04

48

8.3E+05

7.2E+05

8.5E+05

9.7E+05

8.5E+05

8.3E+05

72

2.3E+06

2.2E+06

2.2E+06

2.1E+06

2.2E+06

2.3E+06

Algal density (cells/ml) in control group:

Time (h)

Control group

1

2

3

4

5

6

0

1.0E+04

1.0E+04

1.0E+04

1.0E+04

1.0E+04

1.0E+04

24

6.8E+04

9.2E+04

8.3E+04

8.5E+04

8.7E+04

8.0E+04

48

8.7E+05

8.3E+05

8.2E+05

8.8E+05

7.8E+05

8.2E+05

72

2.4E+06

3.1E+06

2.6E+06

2.8E+06

2.5E+06

2.6E+06

Determination of pH:

pH

Control

Treated

Replica

Test start

Test end

∆pH

Replica

Test start

Test end

∆pH

1

7.78

7.51

0.27

1

6.12

4.98

1.14

2

7.77

7.52

0.25

2

6.13

4.97

1.16

3

7.77

7.54

0.23

3

6.13

5.01

1.12

4

7.78

7.49

0.29

4

6.10

5.01

1.09

5

7.79

7.51

0.28

5

6.13

4.96

1.17

6

7.77

7.48

0.29

6

6.12

4.94

1.18

Temperature recorded

Time (h)

Temperature °C

0

23.0

24

23.1

48

22.9

72

23.1

Validity criteria fulfilled:
yes
Conclusions:
The obtained reuslts in compliance with assay validity criteria, showed that algae EC50 of the nominal concentraiton of the test item '4-aminobenzoyl-b-alanine' after 72 hours is >100 mg/L.

Description of key information

A GLP study was performed in accordance with OECD Guideline 201 to assess the toxicity of the test material, 4 -aminobenzoyl-b-alanine, to algae. According to the results of the test, the EC50 (72 h) was > 100 mg/L (nominal).

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information