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EC number: 616-017-7 | CAS number: 7377-08-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- pH
- Dissociation constant
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Start: 02/07/2018 - End: 09/07/2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green alga
- Strain: SAG 61:81
- Source: laboratory culture
ACCLIMATION
- Acclimation period:
- Culturing media and conditions: Culture medium was kept in 250ml Erlenmeyer flasks in the following conditions:
-Temperature: 23° ± 2°C
- Lighting: lighting greater than 4300 lux
- Sirring: steady with electrical stirrer - Test type:
- static
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- 23 ± 2°C
Refer to results table below - pH:
- Refer to results table below
- Nominal and measured concentrations:
- 100.0 mg/l (nominal concentration)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Initial cells density: 10^4 cells/ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionized water
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Light intensity and quality: Continuous - with flourescent lamp 8000 lux
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 5 ten-fold dilutions from 100 mg/l to 0.01 mg/l.
- Results used to determine the conditions for the definitive study: Yes - Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Validity criteria fulfilled:
- yes
- Conclusions:
- The obtained reuslts in compliance with assay validity criteria, showed that algae EC50 of the nominal concentraiton of the test item '4-aminobenzoyl-b-alanine' after 72 hours is >100 mg/L.
Reference
Stability of the test item:
The test item was considered stable because the measured concentrations of the test item at the end of the test has been maintained within ± 20% at the beginning of the test.
The test item resulted stable during the test, therefore the statistical analysis (EC50) has been performed using the nominal concnetration at T=0.
Nominal conc. |
Measured conc (mg/l) T=0 |
Measured conc. (mg/l) T=72 H |
% concentration variation |
Stability |
Treated |
102.70 |
104.02 |
1.28 |
Stable |
Algal density (cells/ml) in treated group
Time (h) |
Treated group |
|||||
1 |
2 |
3 |
4 |
5 |
6 |
|
0 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
24 |
7.7E+04 |
7.7E+04 |
8.0E+04 |
7.8E+04 |
7.5E+04 |
7.2E+04 |
48 |
8.3E+05 |
7.2E+05 |
8.5E+05 |
9.7E+05 |
8.5E+05 |
8.3E+05 |
72 |
2.3E+06 |
2.2E+06 |
2.2E+06 |
2.1E+06 |
2.2E+06 |
2.3E+06 |
Algal density (cells/ml) in control group:
Time (h) |
Control group |
|||||
1 |
2 |
3 |
4 |
5 |
6 |
|
0 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
1.0E+04 |
24 |
6.8E+04 |
9.2E+04 |
8.3E+04 |
8.5E+04 |
8.7E+04 |
8.0E+04 |
48 |
8.7E+05 |
8.3E+05 |
8.2E+05 |
8.8E+05 |
7.8E+05 |
8.2E+05 |
72 |
2.4E+06 |
3.1E+06 |
2.6E+06 |
2.8E+06 |
2.5E+06 |
2.6E+06 |
Determination of pH:
pH |
|||||||
Control |
Treated |
||||||
Replica |
Test start |
Test end |
∆pH |
Replica |
Test start |
Test end |
∆pH |
1 |
7.78 |
7.51 |
0.27 |
1 |
6.12 |
4.98 |
1.14 |
2 |
7.77 |
7.52 |
0.25 |
2 |
6.13 |
4.97 |
1.16 |
3 |
7.77 |
7.54 |
0.23 |
3 |
6.13 |
5.01 |
1.12 |
4 |
7.78 |
7.49 |
0.29 |
4 |
6.10 |
5.01 |
1.09 |
5 |
7.79 |
7.51 |
0.28 |
5 |
6.13 |
4.96 |
1.17 |
6 |
7.77 |
7.48 |
0.29 |
6 |
6.12 |
4.94 |
1.18 |
Temperature recorded
Time (h) |
Temperature °C |
0 |
23.0 |
24 |
23.1 |
48 |
22.9 |
72 |
23.1 |
Description of key information
A GLP study was performed in accordance with OECD Guideline 201 to assess the toxicity of the test material, 4 -aminobenzoyl-b-alanine, to algae. According to the results of the test, the EC50 (72 h) was > 100 mg/L (nominal).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
Additional information
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