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EC number: 200-059-4 | CAS number: 50-69-1
Mean Tissue Viability in the EpiOcular™ Test with D-Ribose
Mean tissue viability (percentage of control)
Difference between two tissues (percentage)
Individual OD Measurements at 570 Nm
OD570 measurement 1
OD570 measurement 2
OD = Optical density
Duplicate exposures are indicated by A and B.
The objective of this study was to evaluate the eye hazard potential of D-Ribose. For this purpose D-Ribose was topically applied on the Reconstructed Human EpiOcular™ Model.
The possible eye hazard potential of D-Ribose was tested through topical application for 6 hours.
The study procedures described in this report were based on the most recent OECD guideline. Batch Q0926501 of D-Ribose was a white to slightly yellow powder. D-Ribose (55.7 to
58.2 mg) was applied directly on top of the tissue for 6 hours ± 15 minutes.
After exposure the cornea epithelial construct was thoroughly rinsed to remove the test item and transferred to fresh medium for an immersion incubation. Afterwards, the tissues were transferred to fresh medium and incubated for 18 hours at standard culture conditions, prior to determination of the cytotoxic (irritancy) effect.
The positive control had a mean cell viability of 15% after 6 hours ± 15 minutes exposure.The absolute mean OD570(optical density at 570 nm) of the negative control tissues waswithin the laboratory historical control data range. The difference between the percentage of viability of two tissues treated identically was less than 10%, indicating that the test system functioned properly.
Eye hazard potential is expressed as the remaining cell viability after exposure to the test item. The relative mean tissue viability obtained after 6 hours ± 15 minutes treatment with D-Ribose compared to the negative control tissues was 84%. Since the mean relative tissue viability for D-Ribose was above 60% after 6 hours ± 15 minutes treatment D-Ribose is considered to be non-irritant.
In conclusion, D-Ribose is non-irritant in the EpiOcular™ test under the experimental conditions described in this report.
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