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EC number: 200-059-4 | CAS number: 50-69-1
Mean Tissue Viability in the In Vitro Skin Irritation Test with D-Ribose
Table1. Mean absorption in the in vitro skin irritation test with D-Ribose
The objective of this study was to evaluate D-Ribose for its ability to induce skin irritation on a human three dimensional epidermal model (EPISKIN Small model (EPISKIN-SMTM)).
The possible skin irritation potential of
was tested through topical application for 15 minutes.
The study procedures described in this report were based on the most recent OECD and EC guidelines.
Batch Q0926501 of D-Ribose was a white to slightly yellow powder. Skin tissue was moistened with 5 μL of Milli-Q water and at least 10 mg of D-Ribose was applied directly on top of the skin tissue for 15 ± 0.5 minutes. After a 42 hour post-incubation period, determination of the cytotoxic (irritancy) effect was performed. Cytotoxicity is expressed as the reduction of mitochondrial dehydrogenase activity measured by formazan production from MTT at the end of the treatment.
Skin irritation is expressed as the remaining cell viability after exposure to the test item. The relative mean tissue viability obtained after 15 ± 0.5 minutes treatment with D-Ribose compared to the negative control tissues was 111%. Since the mean relative tissue viability for D-Ribose was above 50% after 15 ± 0.5 minutes treatment Riboxyl TM is considered to be non-irritant.
The positive control had a mean cell viability of 7.8% after 15 ± 0.5 minutes exposure. The absolute mean OD570 (optical density at 570 nm) of the negative control tissues was within the laboratory historical control data range. The standard deviation value of the percentage viability of three tissues treated identically was < 10%, indicating that the test system functioned properly.
In conclusion, D-Ribose is non-irritant in the in vitro skin irritation test under the experimental conditions described in this report and should not be classified according to the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) of the United Nations.
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