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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study (OECD), GLP compliance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
COMPLIANCE WITH GOOD LABORATORY PRACTICE I the undersigned declare that the studies described in this report have been conducted under my supervision and in compliance with the following standards of Good Laboratory Practice: Organization for Economic Co-

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
162.2 mg/L
Basis for effect:
mobility

Any other information on results incl. tables

Quality criteria have been satisfied:

% of Daphnie immobilized present in control: 0

 

Analytical verification of the concentration of the test substance

The test substance is considered stable, because the HPLC-UV values, at the beginning and at the

end of the test, at lower and higher concentration, remain within the required range, so results are

referred to the nominal concentration.

The percentage stability for lower concentration is 99%

The percentage stability for higher concentration is 100%

The results are reported in the Appendix 5.

 

EC50

EC50was evaluted using EPA Probit analysis program version1.5.

 

On the basis of results, interpreted according with OECD guideline N° 202, the test substance

“OXALIC ACID DI-HYDRATE” has an EC50(48h) of 162.2 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
no
Conclusions:
The 48h EC50 was 162.2 mg/l
Executive summary:

Daphnia magna were exposed to oxalic acid during 48 h according to the guideline OECD TG 202 in a GLP laboratory test. An 48 h EC50 of 136.9 mg/l was determined.