Registration Dossier
Registration Dossier
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EC number: 819-558-9 | CAS number: 2020359-04-8
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one
- EC Number:
- 819-558-9
- Cas Number:
- 2020359-04-8
- Molecular formula:
- C29H39NO2
- IUPAC Name:
- 1-(9,9-dibutyl-9H-fluoren-2-yl)-2-methyl-2-(morpholin-4-yl)propan-1-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- a off-white powdery solid, without irritating odor
Constituent 1
Study design
- Test type:
- not specified
- Water media type:
- other: tap water
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20.4 – 21.2 °C
- pH:
- 7.0
- Details on test conditions:
- Duration 3 hours
Replicates 1 replicate/concentration (positive control, test item lower concentrations)
5 replicates for test item treatment 1000 mg/L
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water, see annex 2, page 22
Aeration purified air, using Pasteur pipettes
Feeding nutrient solution, 16 mL/vessel
Temperature 20.4 – 21.2 °C - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (CAS-No. 591-35-5)
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- One experiment was performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Because no significant inhibition was observed, no additional experiment had to be per-formed.
All validity criteria were met. For the estimation of the EC50 of the positive control, the fits showed good statistical correspondence of the data with the dose-response-equation. The positive control gave an EC50 of 7.4 mg/L (95 % confidence interval not determinable) which lies within the recommended range of 2 – 25 mg/L. The coefficient of variation of oxygen uptake rate in control replicates was below 30% at the end of the test. The oxygen uptake rate of the blank controls was above 20 mg O2 per gram activated sludge in 1 hour.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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