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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Test material form:
liquid
Specific details on test material used for the study:
1. Preparation of test substance
The test substance was calibrated according to purity (100%). The method of preparation was selected to maximize the solubility of the test substance under specific test exposure conditions. Test substance of 0.05000 and 0.05002 g was dissolved in M4 media as solvent to make the test stock solution. The test substance was prepared in 500 mL capacity glass volumetric flask with 500 mL of dilution agent. Then the volumetric flask was stirred at 200 rpm with a stir bar (38 mm) was added. Test substances were prepared before 48 hours prior to the treatment at room temperature. After stirring for 48 hours, the test stock solution was filtered through 0.45 μm PVDF filter.
2. Analysis of test substance
The analyses of test substance in the test water was not conducted because the analytical method of test substance could not be established.

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
The test substance was calibrated according to purity (100%). The method of preparation was selected to maximize the solubility of the test substance under specific test exposure conditions. Test substance of 0.05000 and 0.05002 g was dissolved in M4 media as solvent to make the test stock solution. The test substance was prepared in 500 mL capacity glass volumetric flask with 500 mL of dilution agent. Then the volumetric flask was stirred at 200 rpm with a stir bar(38 mm) was added. Test substances were prepared before 48 hours prior to the treatment at room temperature. After stirring for 48 hours, the test stock solution was filtered through 0.45 μm PVDF filter.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
1. Age
Less than 24 hours after birth
2. Selection of the test species
The water flea (Daphnia magna) is recommended in “OECD guideline for the testing of chemicals 202”, and was selected for this test because this species have a plenty of comparable toxicological data.
3. Environmental condition
The daphnia were bred in 1 L cylinder glass bath. The temperature of breeding water is maintained within the range of 18~22℃ and photo periods are 16 hours light and 8 hours darkness.
4. Feeding
Feeds were supplied once a day: chlorella vulgaris
5. Breeding water and water quality
The water quality was analyzed for total hardness and alkalinity of the test water was measured by hardness meter and alkalinity meter.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Remarks on exposure duration:
24 and 48 hrs

Test conditions

Hardness:
190 mg/L as CaCO3
Test temperature:
20.7℃
pH:
pH 7.98
Dissolved oxygen:
7.87 mg/L
Nominal and measured concentrations:
100.0 mg/L(nominal concentration)
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOELR
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Details on results:
There was no immobility observed in any of the daphnia exposed to control group and 100.0 mg/L of test solution at 48 hours. No abnormal behavior was observed in control and treatment groups during a limit test.
Reported statistics and error estimates:
EC50 and 95% confidence limits were not statistically calculated, because no immobility was observed after 48 hours in the highest concentration, 100.0 mg/L. Also, effects concentrations in tests based upon WSF (Water Soluble Fraction) was able to calculate from the loading rates and are identified as either EL50 values. NOEC was determined as the highest concentration causing no abnormal behavior and immobility and becomes the NOELR (No Observable Effect Loading Rate). The methods used to determine EL50 and NOELR values was the same as those used to determine EC50 and NOEC values.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
The 48 hours EL50 value of 1,2,3-tris(2,3-epoxypropoxy)propane to Daphnia magna was determined to be higher than 100.0 mg/L, NOELR was 100.0 mg/L. The EL50 and NOELR were based on nominal concentration (loading rate).