(S)-7-hydroxy-3,7-dimethyloctanal

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

other: Annex V to EU Directive 79/831/EEC (4) , C2 method

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test medium of the highest test concentration of nominal 500 mg/l was prepared. Subsequently the stock solution was diluted serially with test water to obtain the desired test concentrations.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours

ACCLIMATION
- Type and amount of food: Green alga culture once daily
- Feeding frequency: daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2 .70 ± 0 .5 mmol/I

Test temperature:

19 ± 1 °C

pH:

6.31 - 8.15

Dissolved oxygen:

8.61 - 9.25 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
0, 31.25, 62.5, 125, 250, 500 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel:flat bottom glasses
- Fill volume: 10 ml
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:

- Ca/mg ratio: 4:1
- Conductivity: 600-700 µSiemens/cm


OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: About 5 microEinstein (m²s) in the wavelength range
of 400-750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 3, 6, 24 and 48 hours

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

410 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

48 h NOEC 250 mg/L (nominal) based on test material
48 h EC0 250 mg/L (nominal) based on test material
48 h EC100 >=500 mg/L (nominal) based on test material

Conclusions:

The EC50 of the short term toxicity on the mobility of Daphnids was determined to be 410 mg/L.

Description of key information

With high probability acutely not harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

410 mg/L

Additional information

The acute toxicity of the test substance to Daphnia magna was examined in a static test conducted according to EU Directive 79/831/EEC. Test organisms of the species Daphnia magna were exposed to the substance for 48 h. At test termination, an EC50 of 410 mg/L (nominal) was determined.