Haematoxylin

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Haematoxylin

EC Number:

208-237-3

EC Name:

Haematoxylin

Cas Number:

517-28-2

Molecular formula:

C16H14O6

IUPAC Name:

haematoxylin

Specific details on test material used for the study:

Hematoxylin / purity of 95%

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Test temperature:

15°C ± 2°C

pH:

7.8

Dissolved oxygen:

99%

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Concentration causing 50% lethality of fish after 96 hours (LC50): > 35 mg/L
No Observed Effect Concentration (NOEC): 35 mg/L

Executive summary:

96h LC50 for fish is > 35 mg/L