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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vivo

Currently viewing:

Administrative data

Endpoint:
in vivo insect germ cell study: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
In vivo genotoxicity assessment of titanium, zirconium and aluminium nanoparticles, and their microparticulated forms, in Drosophila
Author:
Demir, E et al
Year:
2013
Bibliographic source:
Chemosphere Volume 93, Issue 10, November 2013, Pages 2304-2310

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.20 (Sex-linked Recessive Lethal Test in Drosophila melanogaster)
GLP compliance:
not specified
Type of assay:
Drosophila SLRL assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Zirconium dioxide
EC Number:
215-227-2
EC Name:
Zirconium dioxide
Cas Number:
1314-23-4
Molecular formula:
O2Zr
IUPAC Name:
Zirconium dioxide

Test animals

Species:
Drosophila melanogaster
Details on species / strain selection:
Two strains : multiple wing hairs strain with the genetic constitution y; mwh j; and the flare-3 strain with the genetic constitution, flr3/In (3LR) TM3, Bds.
Sex:
male/female

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
Prepared in distilled water
Details on exposure:
Microparticulated 0.1, 1, 5 and 10 mM in feed
Duration of treatment / exposure:
Three day old larvea until pupation
Doses / concentrationsopen allclose all
Dose / conc.:
0.1 other: mM
Dose / conc.:
1 other: mM
Dose / conc.:
5 other: mM
Dose / conc.:
10 other: mM
No. of animals per sex per dose:
Between 10 and 20
Control animals:
yes, concurrent vehicle
other: Positive control ethyl methanesulfonate (EMS)
Positive control(s):
Ethyl methanesulfonate (EMS)

Examinations

Tissues and cell types examined:
Wing spot
Statistics:
Conditional binomial test

Results and discussion

Test results
Sex:
male/female
Genotoxicity:
negative
Toxicity:
no effects
Vehicle controls validity:
valid
Negative controls validity:
not applicable
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Although not following normal guidelines, the outcome of the study considering metal salts is considered valid.